gndA

gndA
168

NADP-dependent phosphogluconate dehydrogenase

Locus
BSU_23860
Molecular weight
51.61 kDa
Isoelectric point
5.08
Protein length
469 aaSequenceBlast
Gene length
1410 bpSequenceBlast
Function
pentose phosphate pathway
Product
NADP-dependent phosphogluconate dehydrogenase
Essential
no
E.C.
1.1.1.44
Synonyms
gndA, yqjI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0362 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,480,750 2,482,159
The protein
Catalyzed reaction/ biological activity
6-phospho-D-gluconate + NADP+ --> CO2 + D-ribulose 5-phosphate + NADPH (according to UniProt)
Protein family
6-phosphogluconate dehydrogenase family (with YqeC and GntZ, according to UniProt)
Structure
2W8Z (PDB) (from Geobacillus stearothermophilus, 83% identity, 92% similarity) PubMed
P80859 (AlphaFold)
Modification
phosphorylation on (Thr-15 OR Thr-17) PubMed
Effectors of protein activity
inhibited by 4-phosphoerythronate (results from oxidation of erythrose-4-phosphate) PubMed
Kinetic information
Reversible reaction PubMed
Paralogous protein(s)
GntZ
Additional information
It contains a cysteine on the active site. The enzyme is a dimer. PubMed
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
gndA
Description
PubMed
Open in new tab

gndA

2025-03-14 18:57:18

Jstuelk

82

95401f88c699f1568c284e161c6e78f59b4fa3db

208B95053B22069BF9C0E4E4BD18072A81E37811

Genes
polY1-gndA
Description
PubMed
Regulation
constitutively expressed PubMed
Open in new tab

polY1→gndA

2025-03-17 17:59:28

Jstuelk

93

0ab6586973869a31966395fe1150bbd523f0e616

DDC038A113D7CE7BB6CF11557454A59E525E06B8

Biological materials
Mutant
MGNA-C391 (yqjI::erm), available at the NBRP B. subtilis, Japan
GP1514 (gndA::kan), available in Jrg Stlke's lab
BKE23860 (gndA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCTAACGTCCCTTCT, downstream forward: _UP4_TAATCAATAGAAACCCCCGA
BKK23860 (gndA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCTAACGTCCCTTCT, downstream forward: _UP4_TAATCAATAGAAACCCCCGA
Expression vectors
pGP1777 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jrg Stlke's lab)
pGP1789 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP1389) (available in Jrg Stlke's lab)
GP1408 (gndA-Strep (spc)) & GP1410 (gndA-Strep (cat)), purification from B. subtilis, for SPINE, available in Jrg Stlke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab
FLAG-tag construct
GP1403 (spc, based on pGP1331), available in Jrg Stlke's lab
GP1408 (kan, resistance cassette exchange in GP1403), available in Jrg Stlke's lab
References
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61B7C51EB7E74226890010B61D8E41C02189A453

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Time of last update: 2025-04-05 19:44:09

Author of last update: Jstuelk