ispH
168

(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 7th step in the MEP pathway of isoprenoid biosynthesis

Locus

BSU_25160

Molecular weight

34.77 kDa

Isoelectric point

5.61

Protein length

314 aaSequenceBlast

Gene length

945 bpSequenceBlast

Function

MEP pathway of isoprenoid biosynthesis

Product

(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Essential

yes

E.C.

1.17.1.2

Synonyms

ispH, yqfP

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0761 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

2,596,535 2,597,479

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

H₂O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] --> (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H⁺ + 2 reduced [2Fe-2S]-[ferredoxin] (according to UniProt)

dimethylallyl diphosphate + H₂O + 2 oxidized [2Fe-2S]-[ferredoxin] --> (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H⁺ + 2 reduced [2Fe-2S]-[ferredoxin] (according to UniProt)

Protein family

ispH family (single member, according to UniProt)

Cofactors

Fe-S cluster PubMed

Structure

3F7T (PDB) (IspH from E. coli, 38% identity) PubMed

P54473 (AlphaFold)

Expression and Regulation

Operons

Genes

yqfQ-ispH

Description