ylmB

ylmB
168

N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase, thiamine salvage pathway

Locus
BSU_15350
Molecular weight
47.02 kDa
Isoelectric point
4.98
Protein length
426 aaSequenceBlast
Gene length
1281 bpSequenceBlast
Function
thiamine salvage
Product
N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase
Essential
no
Synonyms
ylmB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0624 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,607,556  1,608,836
The protein
Catalyzed reaction/ biological activity
H2O + N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine --> 4-amino-5-aminomethyl-2-methylpyrimidine + formate (according to UniProt)
Protein family
peptidase M20A family (according to UniProt)
Cofactors
Binds 2 zinc or cobalt ions per subunit (according to Swiss-Prot)
Structure
3PFO (PDB) (from Rhodopseudomonas palustris, 25% identity)
O31724 (AlphaFold)
Paralogous protein(s)
YodQ
Expression and Regulation
Operons
Genes
ylmB
Description
PubMed
Regulatory mechanism
Thi-box: RNA switch, antitermination via RNA switch, in Thi-box regulon
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ylmB

2025-03-16 12:03:41

Jstuelk

84

eb1bd6149c87939790dcca836b55754c23c7618e

672F2266806F302505E82A023D8EC8575EC8A721

Biological materials
Mutant
MGNA-B124 (ylmB::erm), available at the NBRP B. subtilis, Japan
BKE15350 (ylmB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGTAAATCCGCTCCTAT,  downstream forward: _UP4_TGACATGAAAATTTCTTCTT
BKK15350 (ylmB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGTAAATCCGCTCCTAT,  downstream forward: _UP4_TGACATGAAAATTTCTTCTT
References
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Page visits: 4248

Time of last update: 2025-04-06 10:59:00

Author of last update: Melvin.boenninger