thiD

thiD
168

4-amino-5-hydroxymethyl-2-methylpyrimidine and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase

Locus
BSU_11710
Molecular weight
28.97 kDa
Isoelectric point
5.71
Protein length
271 aaSequenceBlast
Gene length
816 bpSequenceBlast
Function
biosynthesis of thiamine pyrophosphate (TPP)
Product
4-amino-5-hydroxymethyl-2-methylpyrimidinepyrophosphate kinase
Essential
no
E.C.
2.7.1.49
Synonyms
thiD, yjbV
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0351 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,246,837  1,247,652
The protein
Catalyzed reaction/ biological activity
4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP --> 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ADP + H+ (according to UniProt)
4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP --> 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP (according to UniProt)
Protein family
ThiD family (with PdxK, according to UniProt)
Structure
2I5B (PDB) PubMed
O31620 (AlphaFold)
Paralogous protein(s)
PdxK
Expression and Regulation
Operons
Genes
tenA-tenI-thiO-thiS-thiG-thiF-thiD
Description
PubMed
Regulation
repressed by thiamine (Thi-box) PubMed
antitermination is modulated by NusG-dependent transcription pausing just upstream of the Thi-box PubMed
the Thi-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
Thi-box: RNA switch, via RNA switch, in Thi-box regulon
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tenA→thiD

2025-04-03 13:11:27

Jstuelk

127

A48634F4963321F6F5F59C5107F472325227A1B0

A1FEA45B91FD65F137EB4D1A0FCD88BC91331EAF

Biological materials
Mutant
MGNA-B167 (yjbV::erm), available at the NBRP B. subtilis, Japan
BKE11710 (thiD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACTCATTACACTACACTCCT,  downstream forward: _UP4_TGAGATCACCAGCTGATGGT
BKK11710 (thiD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACTCATTACACTACACTCCT,  downstream forward: _UP4_TGAGATCACCAGCTGATGGT
References
Reviews
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Original Publications
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97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB

Page visits: 3322

Time of last update: 2025-04-04 13:15:25

Author of last update: Melvin.boenninger