srtN

srtN
168

protein deacetylase and lipoamidase

Locus
BSU_09650
Molecular weight
27.27 kDa
Isoelectric point
5.13
Protein length
247 aaSequenceBlast
Gene length
744 bpSequenceBlast
Function
control of AcsA and MenE activities
Product
NAD(+)-dependent deacetylase and lipoamidase
Essential
no
Synonyms
srtN, yhdZ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0846 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,040,094  1,040,837
Phenotypes of a mutant
increased 2-oxoglutarate dehydrogenase activity PubMed
The protein
Catalyzed reaction/ biological activity
H2O + N6-acetyl-L-lysyl-[protein] + NAD+ --> 2''-O-acetyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide (according to UniProt)
deacetylates (and thereby activates) AcsA (together with AcuC) PubMed
deacetylates (and thereby activates) MenE PubMed
removes lipoic acid from E2 subunits of ketoacid dehydrogenases and from the H protein of the glycine cleavage system PubMed
Protein family
sirtuin family (single member, according to UniProt)
Domains
Deacetylase sirtuin-type domain (aa 2-247) (according to UniProt)
Structure
1MA3 (PDB) (from Archaeoglobus fulgidus, 40% identity) PubMed
O07595 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
srtN
Description
PubMed
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srtN

2025-04-03 05:25:25

Jstuelk

76

30880a48897d707c124222775226c50c7f79f3e3

E6861320780F3842D35EACA562232A7CAFCC92CA

Biological materials
Mutant
MGNA-B488 (yhdZ::erm), available at the NBRP B. subtilis, Japan
GP1210 (''srtN::cat''), available in Jörg Stülke's lab
1A888 ( srtN::spec), PubMed, available at BGSC
BKE09650 (srtN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACTCAACACCGCCTTTTT,  downstream forward: _UP4_TGAGCCGCTTTATGCGCCTC
BKK09650 (srtN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACTCAACACCGCCTTTTT,  downstream forward: _UP4_TGAGCCGCTTTATGCGCCTC
References
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Page visits: 4835

Time of last update: 2025-04-05 21:16:53

Author of last update: Jstuelk