xpt

xpt
168

xanthine phosphoribosyltransferase

Locus
BSU_22070
Molecular weight
20.89 kDa
Isoelectric point
5.84
Protein length
194 aaSequenceBlast
Gene length
585 bpSequenceBlast
Function
purine salvage and interconversion
Product
xanthine phosphoribosyltransferase
Essential
no
E.C.
2.4.2.22
Synonyms
xpt
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0503 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,319,440 → 2,320,024
The protein
Catalyzed reaction/ biological activity
5-phospho-α-D-ribose 1-diphosphate + xanthine --> diphosphate + XMP (according to UniProt)
Protein family
Purine/pyrimidine phosphoribosyltransferase family (according to UniProt)
Structure
1Y0B (PDB),  2FXV (PDB) (complex with GMP), 1Y27 (PDB) (the riboswitch in complex with guanine)
P42085 (AlphaFold)
Effectors of protein activity
strongly inhibited by pGpp and ppGpp PubMed
weakly inhibited by pppGpp PubMed
Localization
cytoplasm (according to Swiss-Prot)
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and Regulation
Operons
Genes
xpt-pbuX
Description
PubMed
Regulation
induced in the absence of guanine (G-box) PubMed
Regulatory mechanism
G-box: RNA switch, riboswitch PubMed, in G-box
PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

xpt→pbuX

2025-04-01 19:22:59

Jstuelk

112

e2aea9be4482d2f1a4ec3d17e057d345c5db5dbf

D9AD2007FF8AC8985A79EBB6FAAF4F457CB0852B

Biological materials
Mutant
MGNA-A870 (xpt::erm), available at the NBRP B. subtilis, Japan
BKE22070 (xpt::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCTGTCTACCTCCGTTA,  downstream forward: _UP4_TCCTTCGTACAGGAGGTTCA
BKK22070 (xpt::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCTGTCTACCTCCGTTA,  downstream forward: _UP4_TCCTTCGTACAGGAGGTTCA
LacZ fusion
pGP430 (in pAC7), available in Jörg Stülke's lab
References
Loading

02C84C9A8C0008CC253CBF13584A4B5B2D2B510C

Page visits: 4872

Time of last update: 2025-04-06 05:24:34

Author of last update: Jstuelk