thrS

thrS
168

threonyl-tRNA synthetase (major)

Locus
BSU_28950
Molecular weight
73.34 kDa
Isoelectric point
5.21
Protein length
643 aaSequenceBlast
Gene length
1932 bpSequenceBlast
Function
translation
Product
threonyl-tRNA synthetase (major)
Essential
no
E.C.
6.1.1.3
Synonyms
thrS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0441 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,959,257 2,961,188
The protein
Catalyzed reaction/ biological activity
ATP + L-threonine + tRNAThr --> AMP + diphosphate + H+ + L-threonyl-tRNAThr (according to UniProt)
Protein family
Class-II aminoacyl-tRNA synthetase family (according to UniProt)
Domains
TGS domain (aa 3-64) (according to UniProt)
Structure
1TJE (PDB) (from Escherichia coli, 44% identity, 63% similarity) PubMed
P18255 (AlphaFold)
Modification
Cys573 is S-bacillithiolated by NaOCl stress PubMed
Paralogous protein(s)
ThrZ, one of the two proteins has to be present for viability PubMed
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
thrS
Description
PubMed
Regulation
expression transiently increases in the forespore PubMed
the T-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
T-box: RNA switch, in T-box
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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thrS

2025-03-16 16:35:13

Jstuelk

67

a985747c3039290069594fa9e15bc4414dff3f42

ADDA3449A78D3CEE70744E42A28DF865BB949B42

Genes
dnaB-dnaI-ytxB-ytxC-thrS
Description
PubMed
Open in new tab

dnaB→thrS

2025-03-17 19:32:17

Jstuelk

136

86944789c9fccd66104bd44b9e445a3dc0b7ffc0

B3B673302E6466DA20A850B2B832501BE5C0DACF

Biological materials
Mutant
BKE28950 (thrS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTGTCACTCCTTTTT, downstream forward: _UP4_TAAAATAAAAAAGCATGATC
BKK28950 (thrS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTGTCACTCCTTTTT, downstream forward: _UP4_TAAAATAAAAAAGCATGATC
References
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3E1131CFA3EDEB3865638F09BE2F6B6ED2570BE2

Page visits: 4837

Time of last update: 2025-04-06 08:20:25

Author of last update: Melvin.boenninger