tgt

tgt
168

tRNA-guanine transglycosylase

Locus
BSU_27710
Molecular weight
43.55 kDa
Isoelectric point
6.15
Protein length
Gene length
Function
tRNA modification
Product
tRNA-guanine transglycosylase
Essential
no
E.C.
2.4.2.29
Synonyms
tgt

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0343 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,832,727  2,833,872
The protein
Catalyzed reaction/ biological activity
7-aminomethyl-7-carbaguanine + guanosine34 in tRNA --> 7-aminomethyl-7-carbaguanosine34 in tRNA + guanine (according to UniProt)
Protein family
queuine tRNA-ribosyltransferase family (single member, according to UniProt)
Structure
1PUD (PDB) (from Zymomonas mobilis, 52% identity) PubMed
cytosol(according to UniProt)
Expression and Regulation
Operons
Description
Regulation
the mRNA is processed between tgt and yrbF by RNase Y, this requires the RicA-RicF-RicT complex PubMed
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ruvAyrbF

2025-01-10 18:20:36

Jstuelk

107

b67b31b8062f65eb2771aa03f56aa1b45473760c

1495BBE3D6ACD7DA206B13AA8930F5C46B2B7309

Biological materials
Mutant
BKE27710 (tgt::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CACTTTTGATGAGGCCTCCT,  downstream forward: _UP4_TAAATAAACATAACAGGCTT
BKK27710 (tgt::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CACTTTTGATGAGGCCTCCT,  downstream forward: _UP4_TAAATAAACATAACAGGCTT
References
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0383C8214ADE2FF70CFDB953A65648B37A9D3216

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Time of last update: 2025-01-22 16:34:55

Author of last update: Jstuelk