pyrAB

pyrAB
168

carbamoyl-P synthetase (catalytic subunit)

Locus
BSU_15520
Molecular weight
117.44 kDa
Isoelectric point
4.78
Protein length
1071 aaSequenceBlast
Gene length
3216 bpSequenceBlast
Function
pyrimidine biosynthesis
Product
carbamoyl-P synthetase (catalytic subunit)
Essential
no
E.C.
6.3.5.5
Synonyms
pyrAB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0458 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,623,736 → 1,626,951
The protein
Catalyzed reaction/ biological activity
2 ATP + H2O + bicarbonate + L-glutamine --> 2 ADP + carbamoyl-P + 2 H+ + L-glutamate + phosphate (according to UniProt)
Protein family
carB family (with CarB, according to UniProt)
Domains
2 ATP-grasp domains (aa 133-327, aa 671-861) (according to UniProt)
MGS-like domain (aa 930-1071) (according to UniProt)
Structure
1JDB (PDB) (from Escherichia coli, 49% identity, 69% similarity) PubMed
P25994 (AlphaFold)
Paralogous protein(s)
CarB
Localization
membrane PubMed
Expression and Regulation
Operons
Genes
pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE
Description
PubMed
Regulation
induced in the absence of uridine nucleotides (PyrR) PubMed
Regulatory mechanism
PyrR: termination, via RNA switch, in pyrR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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pyrR→pyrE

2025-04-07 02:33:30

ghost

112

3b978222b0b6a5230a2e7701ac35d1e35cc85307

0A8FD5CB6489EDD8CB2074130B763DCAC1F24654

Biological materials
Mutant
BKE15520 (ΔpyrAB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATTACTAAAATTTTGTTAA,  downstream forward: _UP4_AATCAGGAGGCGGCAGTCAC
BKK15520 (ΔpyrAB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATTACTAAAATTTTGTTAA,  downstream forward: _UP4_AATCAGGAGGCGGCAGTCAC
GP3717 (pyrAB::aphA3 trpC2) available in the Stülke lab
GP3719 (pyrAAAB::aphA3 trpC2) available in the Stülke lab
GP3721 (pyrAAAB::cat trpC2) available in the Stülke lab
References
Reviews
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Original Publications
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D57DC8A53E52BC4A2EA894E3C9FD983592882918

Page visits: 3370

Time of last update: 2025-04-25 22:33:06

Author of last update: Jstuelk