purK

purK
168

phosphoribosylaminoimidazole carboxylase (ATP-dependent)

Locus
BSU_06430
Molecular weight
42.00 kDa
Isoelectric point
5.12
Protein length
380 aaSequenceBlast
Gene length
1143 bpSequenceBlast
Function
purine biosynthesis
Product
phosphoribosylaminoimidazole carboxylase (ATP-dependent)
Essential
no
E.C.
4.1.1.21
Synonyms
purK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0026 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
699,093  700,235
The protein
Catalyzed reaction/ biological activity
5-amino-1-(5-phospho-β-D-ribosyl)imidazole + ATP + hydrogencarbonate --> 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + 2 H+ + phosphate(according to UniProt)
Protein family
PurK/PurT family (with PurT, according to UniProt)
Domains
ATP-grasp doamin (aa 112-298) (according to UniProt)
Structure
2Z04 (PDB) (from Aquifex aeolicus, 37% identity, 57% similarity)
P12045 (AlphaFold)
Paralogous protein(s)
PurT
Expression and Regulation
Operons
Genes
purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD
Description
PubMed
Regulation
expression activated by glucose (4.4 fold) PubMed
Regulatory mechanism
G-box: RNA switch, (riboswitch) PubMed, in G-box
PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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purE→purD

2025-04-06 02:18:42

Jstuelk

231

ace69dc80f4f087486c218727fa370a7a9630d21

EDD8EC37B0E5B38941D5827C265AB2F23CF23EA7

Biological materials
Mutant
BKE06430 (purK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TACAGCTCCCGGATAGATGA,  downstream forward: _UP4_AATAGAGACGGAGGACAAGC
BKK06430 (purK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TACAGCTCCCGGATAGATGA,  downstream forward: _UP4_AATAGAGACGGAGGACAAGC
References
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Time of last update: 2025-04-06 12:23:04

Author of last update: Melvin.boenninger