pabA

pabA
168

para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II), glutaminase subunit

Locus
BSU_00750
Molecular weight
21.54 kDa
Isoelectric point
4.78
Protein length
194 aaSequenceBlast
Gene length
585 bpSequenceBlast
Function
biosynthesis of folate and tryptophan
Product
para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II) glutamine amidotransferase (subunit B) and anthranilate synthase (subunit II)
Essential
no
E.C.
2.6.1.85
Synonyms
pabA, trpG, trpX
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0512 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon

Gene
Coordinates
84,290  84,874
The protein
Catalyzed reaction/ biological activity
chorismate + L-glutamine --> anthranilate + pyruvate + L-glutamate (according to UniProt)
Domains
Glutamine amidotransferase type-1 domain (aa 1-194) (according to UniProt)
Structure
6QUR (PDB) (synthetic construct, 72.9% identity) PubMed
1I1Q (PDB) (from Salmonella typhimurium, 42% identity) PubMed
P28819 (AlphaFold)
Expression and Regulation
Operons
Genes
pabB-pabA-pabC-sul-folB-folK-yazB-dusB1-lysS
Description
PubMed
Regulation
translation repressed by tryptophan (MtrB) PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

pabB→lysS

2025-04-04 11:44:49

Jstuelk

121

8c465dd3eec1aaa32d5e91206834f9bb99ef474a

7B9041CE94396BF42D285F7C0EDD3A2544761EEE

Other regulations
MtrB: translation inhibition, PubMed
Additional information
the pabA-pabC part of the mRNA is substantially stabilized upon depletion of RNase Y PubMed
Biological materials
Mutant
BKE00750 (pabA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAAATCATTTCTCCGC,  downstream forward: _UP4_TATCGCAAGGAAGTTATTGC
BKK00750 (pabA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAAATCATTTCTCCGC,  downstream forward: _UP4_TATCGCAAGGAAGTTATTGC
References
Reviews
Babitzke P, Baker CS, Romeo T Regulation of translation initiation by RNA binding proteins. Annual review of microbiology. 2009; 63:27-44. doi:10.1146/annurev.micro.091208.073514. PMID:19385727
Gollnick P, Babitzke P, Antson A, Yanofsky C Complexity in regulation of tryptophan biosynthesis in Bacillus subtilis. Annual review of genetics. 2005; 39:47-68. . PMID:16285852
Original Publications
Semmelmann F, Straub K, Nazet J, Rajendran C, Merkl R, Sterner RMapping the Allosteric Communication Network of Aminodeoxychorismate Synthase.Journal of molecular biology. 2019 Jul 12; 431(15):2718-2728. PMID: 31121180
Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. Molecular microbiology. 2011 Sep; 81(6):1459-73. doi:10.1111/j.1365-2958.2011.07777.x. PMID:21815947
Yakhnin H, Yakhnin AV, Babitzke P Translation control of trpG from transcripts originating from the folate operon promoter of Bacillus subtilis is influenced by translation-mediated displacement of bound TRAP, while translation control of transcripts originating from a newly identified trpG promoter is not. Journal of bacteriology. 2007 Feb; 189(3):872-9. . PMID:17114263
Morollo AA, Eck MJ Structure of the cooperative allosteric anthranilate synthase from Salmonella typhimurium. Nature structural biology. 2001 Mar; 8(3):243-7. . PMID:11224570
de Saizieu A, Vankan P, Vockler C, van Loon AP The trp RNA-binding attenuation protein (TRAP) regulates the steady-state levels of transcripts of the Bacillus subtilis folate operon. Microbiology (Reading, England). 1997 Mar; 143 ( Pt 3):979-89. . PMID:9084182
Baumann C, Otridge J, Gollnick P Kinetic and thermodynamic analysis of the interaction between TRAP (trp RNA-binding attenuation protein) of Bacillus subtilis and trp leader RNA. The Journal of biological chemistry. 1996 May 24; 271(21):12269-74. . PMID:8647825
Babitzke P, Stults JT, Shire SJ, Yanofsky C TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis, is a multisubunit complex that appears to recognize G/UAG repeats in the trpEDCFBA and trpG transcripts. The Journal of biological chemistry. 1994 Jun 17; 269(24):16597-604. . PMID:7515880

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Time of last update: 2025-04-06 21:21:08

Author of last update: Jstuelk