odhB

odhB
168

2-oxoglutarate dehydrogenase complex (dihydrolipoamide transsuccinylase, E2 subunit)

Locus
BSU_19360
Molecular weight
45.83 kDa
Isoelectric point
4.86
Protein length
417 aaSequenceBlast
Gene length
1254 bpSequenceBlast
Function
TCA cycle
Product
2-oxoglutarate dehydrogenase complex
Essential
no
E.C.
2.3.1.61
Synonyms
odhB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0508 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,107,505  2,108,758
The protein
Catalyzed reaction/ biological activity
(R)-N6-dihydrolipoyl-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA --> (R)-N6-(S8-succinyldihydrolipoyl)-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + CoA (according to UniProt)
Protein family
2-oxoacid dehydrogenase family (according to UniProt)
Domains
Lipoyl-binding domain (aa 1-76) (according to UniProt)
Peripheral subunit-binding domain (PSBD) (aa 123-160) (according to UniProt)
Cofactors
lipoic acid (on Lys-42), can be removed by SrtN PubMed
Structure
3DUF (PDB) (EI of the PDH complex from Geobacillus stearothermophilus, 38% identity) PubMed
P16263 (AlphaFold)
Modification
phosphorylated on several Arg residues PubMed
Paralogous protein(s)
BkdB, PdhC, AcoC
Localization
membrane associated PubMed
Additional information
extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at Proteopedia
Expression and Regulation
Operons
Genes
odhA-odhB
Description
PubMed
Regulation
repressed by glucose (2.4-fold) (CcpA) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

odhA→odhB

2025-03-21 04:54:23

ghost

103

998628ac6a5b555702d5fa0febcedb0378ac763b

87DE9AE10510310314B50140AE66E41B4448BC3A

Biological materials
Mutant
GP4471 (trpC2 ΔodhB::neo), available in Jörg Stülke's lab
GP1276 Δ(''odhA-odhB)::cat'', available in Jörg Stülke's lab PubMed
GP2332 Δ(odhA-odhB)::kan, Cre-recombinase is integrated in ''sacA'', available in Jörg Stülke's lab
GP2334 Δ(odhA-odhB)::lox72, Cre-recombinase is integrated in ''sacA'', available in Jörg Stülke's lab
BKE19360 (odhB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCCGCCATTTTTTCATTTC,  downstream forward: _UP4_TAATAAAAAAGGGTACATCA
BKK19360 (odhB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCCGCCATTTTTTCATTTC,  downstream forward: _UP4_TAATAAAAAAGGGTACATCA
Expression vectors
pGP1145 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab) PubMed
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab PubMed
FLAG-tag construct
GP1424 (spc, based on pGP1331), available in the Stülke lab
References
Reviews
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Original Publications
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02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2

Page visits: 6418

Time of last update: 2025-04-07 02:32:27

Author of last update: Robert.warneke