hprT

hprT
168

trigger enzyme, hypoxanthine phosphoribosyltransferase and part of a transcription activator

Locus
BSU_00680
Molecular weight
20.10 kDa
Isoelectric point
4.6
Protein length
180 aaSequenceBlast
Gene length
543 bpSequenceBlast
Function
purine salvage and interconversion, control of ftsH expression
Product
hypoxanthine phosphoribosyltransferase
Essential
no
E.C.
2.4.2.8
Synonyms
hprT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0634 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
76,344 → 76,886
Phenotypes of a mutant
essential PubMed, non-essential according to PubMed
inactivation of hprT can overcome the toxicity of guanosine to a rel sasA sasB triple mutant PubMed
The protein
Catalyzed reaction/ biological activity
diphosphate + IMP --> 5-phospho-α-D-ribose 1-diphosphate + hypoxanthine (according to UniProt)
diphosphate + GMP --> 5-phospho-α-D-ribose 1-diphosphate + guanine (according to UniProt)
Protein family
Purine/pyrimidine phosphoribosyltransferase family (according to UniProt)
Structure
6D9Q (PDB) (apo-protein) PubMed
6D9R (PDB) (complex with the substrate PRPP) PubMed
6D9S (PDB) (complex with ppGpp) PubMed
P37472 (AlphaFold)
Effectors of protein activity
inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´, (p)ppGpp binds to the acive site of the enzyme and prevents the conversion of the apo-tetramer to the active dimer PubMed
Kinetic information
KM (PRPP):  166 µM PubMed
KI (pppGpp): 1.7 µM PubMed
Localization
cytoplasm (according to UniProt)
Expression and Regulation
Operons
Genes
tilS-hprT-ftsH
Description
PubMed
Regulation
induced by heat shock (class III)
Regulatory mechanism
TilS: activation, with HprT PubMed, in tilS regulon
HprT: activation, with TilS PubMed, in hprT regulon
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
SigA: sigma factor, PubMed, in sigA regulon
Additional information
the mRNA is very stable (half-life > 15 min) PubMed
Open in new tab

tilS→ftsH

2025-04-04 02:37:40

ghost

102

0b831aee45fd6cc4e3e06cbf53fe7330f12b4cb1

0B2B3D2B74921E6EEF5704482CF1D3DF309FA3D3

Genes
spoIIE-yabS-yabT-tilS-hprT-ftsH
Description
PubMed
Regulation
expressed early during sporulation in the forespore (SigF) PubMed
Sigma factors
SigF: sigma factor, PubMed, in sigF regulon
Open in new tab

spoIIE->ftsH

2025-04-03 08:25:04

Jstuelk

122

7069328a463a18ae1e3c27f03ddf76f79efe7c06

8FBB4B976CF46DAD9437300BBE1B22B618D33ED0

Biological materials
Mutant
BKE00680 (ΔhprT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATGATTTTGCTTGCCC,  downstream forward: _UP4_TGATCGGCAGCCTGCTTCCG
BKK00680 (ΔhprT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATGATTTTGCTTGCCC,  downstream forward: _UP4_TGATCGGCAGCCTGCTTCCG
References
Reviews
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Original Publications
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B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738

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Time of last update: 2025-04-06 20:19:37

Author of last update: Jstuelk