hprK

hprK
168

HPr kinase/ phosphorylase

Locus
BSU_35000
Molecular weight
34.55 kDa
Isoelectric point
4.91
Protein length
310 aaSequenceBlast
Gene length
933 bpSequenceBlast
Function
carbon catabolite repression,phosphorylation of HPr and Crh proteins at Ser46
Product
HPr kinase/ phosphorylase
Essential
no
Synonyms
hprK, ptsK, yvoB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1493 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,594,242  3,595,174
Phenotypes of a mutant
no carbon catabolite repression PubMed
delay in spore germination PubMed
The protein
Catalyzed reaction/ biological activity
[HPr protein]-L-serine + ATP --> [HPr protein]-O-phospho-L-serine + ADP + H+ (according to UniProt)
[HPr protein]-O-phospho-L-serine + H+ + phosphate --> [HPr protein]-L-serine + diphosphate (according to UniProt)
Protein family
HPrK/P family (single member, according to UniProt)
Structure
1KNX (PDB) (from Mycoplasma pneumoniae) PubMed
1KKM (PDB) (complex of Lactobacillus casei HprK with B. subtilis HPr-Ser-P)
1KKL (PDB) (complex of Lactobacillus casei HprK with B. subtilis HPr)
O34483 (AlphaFold)
Expression and Regulation
Operons
Genes
hprK-lgt-yvoD-yvoE-yvoF
Description
PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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hprK→yvoF

2025-04-25 07:54:54

ghost

118

93fddf316d690cb3235f223214494fee7a44414b

CC05676E9E34D6CA810B7283606A4417B30651B3

Biological materials
Mutant
MGNA-A328 (hprK::erm), available at the NBRP B. subtilis, Japan
GP202 (ΔhprK::spc) PubMed, available in Jörg Stülke's lab
GP858 (ΔhprK::aphA3) PubMed, available in Jörg Stülke's lab
GP82 (ΔhprK::cat) PubMed, available in Jörg Stülke's lab
BKE35000 (ΔhprK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGATATGTTCCTCCTGTT,  downstream forward: _UP4_CAAGAAGAATAGGAGTTGGC
BKK35000 (ΔhprK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGATATGTTCCTCCTGTT,  downstream forward: _UP4_CAAGAAGAATAGGAGTTGGC
Expression vectors
for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP642, available in Jörg Stülke's lab
for expression/ purification of mutant HprK-G158A from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP650, available in Jörg Stülke's lab
for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP205, available in Jörg Stülke's lab PubMed
for expression, purification of the N-terminal domain in E. coli with N-terminal His-tag, in pWH844: pGP218, available in Jörg Stülke's lab
LacZ fusion
pGP201 (in pAC5, PubMed) available in Jörg Stülke's lab
pGP202 (in pAC6, PubMed) available in Jörg Stülke's lab
Antibody
available in Jörg Stülke's lab PubMed
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Anne Galinier, University of Marseille, France
References
Reviews
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Structural Analysis of HPrK
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General Analysis, Physiology
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Enzymatic Properties, Mutation Analysis
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HprK as a Target For Antimicrobial Compounds
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771F205F818A755A661B8E1C95365A4F6AEB05C7

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Time of last update: 2025-04-25 15:00:13

Author of last update: Jstuelk