fabI

fabI
168

enoyl-acyl carrier protein reductase

Locus
BSU_11720
Molecular weight
27.72 kDa
Isoelectric point
5.61
Protein length
258 aaSequenceBlast
Gene length
777 bpSequenceBlast
Function
fatty acid biosynthesis
Product
enoyl-acyl carrier protein reductase
Essential
no
E.C.
1.3.1.9
Synonyms
fabI, yjbW
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0623 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,247,788 1,248,564
The protein
Catalyzed reaction/ biological activity
2,3-saturated acyl-[ACP] + NAD+ --> (2E)-enoyl-[ACP] + H+ + NADH (according to UniProt)
Protein family
Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)
Structure
3OIF (PDB) (complex with NAD and inhibitor triclosan) PubMed
P54616 (AlphaFold)
Effectors of protein activity
inhibited by triclosan PubMed
Paralogous protein(s)
FabL, one of the two proteins has to be present for viability PubMed
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
fabI
Description
PubMed
Regulation
expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
Regulatory mechanism
FapR: repression, PubMed, in fapR regulon
Sigma factors
SigI: sigma factor, PubMed, in sigI regulon
Open in new tab

fabI

2025-03-10 19:23:32

Jstuelk

74

e895538eabe45648d8e47580c56c75c9e764a2d2

941499B9D8F24ADDB5F3EDF54158C28DCE6192A6

Biological materials
Mutant
MGNA-B168 (yjbW::erm), available at the NBRP B. subtilis, Japan
BKE11720 (fabI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTATAAAGCCTCCTA, downstream forward: _UP4_TAAGCAGGATCTATATCAAG
BKK11720 (fabI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTATAAAGCCTCCTA, downstream forward: _UP4_TAAGCAGGATCTATATCAAG
References
Reviews
Fujita Y, Matsuoka H, Hirooka K Regulation of fatty acid metabolism in bacteria. Molecular microbiology. 2007 Nov; 66(4):829-39. . PMID:17919287
White SW, Zheng J, Zhang YM, Rock The structural biology of type II fatty acid biosynthesis. Annual review of biochemistry. 2005; 74:791-831. . PMID:15952903
Original Publications
Pisithkul T, Schroeder JW, Trujillo EA, Yeesin P, Stevenson DM, Chaiamarit T, Coon JJ, Wang JD, Amador-Noguez D Metabolic Remodeling during Biofilm Development of Bacillus subtilis. mBio. 2019 May 21; 10(3). pii:e00623-19. doi:10.1128/mBio.00623-19. PMID:31113899
Ramaniuk O, Převorovský M, Pospíšil J, Vítovská D, Kofroňová O, Benada O, Schwarz M, Šanderová H, Hnilicová J, Krásný L σ from : Impact on Gene Expression and Characterization of σ-dependent Transcription that Requires New Types of Promoters with Extended -35 and -10 Elements. Journal of bacteriology. 2018 Jun 18; . pii:JB.00251-18. doi:10.1128/JB.00251-18. PMID:29914988
Wenzel M, Patra M, Albrecht D, Chen DY, Nicolaou KC, Metzler-Nolte N, Bandow JE Proteomic signature of fatty acid biosynthesis inhibition available for in vivo mechanism-of-action studies. Antimicrobial agents and chemotherapy. 2011 Jun; 55(6):2590-6. doi:10.1128/AAC.00078-11. PMID:21383089
Kim KH, Ha BH, Kim SJ, Hong SK, Hwang KY, Kim EE Crystal structures of Enoyl-ACP reductases I (FabI) and III (FabL) from B. subtilis. Journal of molecular biology. 2011 Feb 25; 406(3):403-15. doi:10.1016/j.jmb.2010.12.003. PMID:21185310
Thomaides HB, Davison EJ, Burston L, Johnson H, Brown DR, Hunt AC, Errington J, Czaplewski L Essential bacterial functions encoded by gene pairs. Journal of bacteriology. 2007 Jan; 189(2):591-602. . PMID:17114254
Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M A comprehensive proteome map of growing Bacillus subtilis cells. Proteomics. 2004 Oct; 4(10):2849-76. . PMID:15378759
Schujman GE, Paoletti L, Grossman AD, de Mendoza D FapR, a bacterial transcription factor involved in global regulation of membrane lipid biosynthesis. Developmental cell. 2003 May; 4(5):663-72. . PMID:12737802
Heath RJ, Su N, Murphy CK, Rock CO The enoyl-[acyl-carrier-protein] reductases FabI and FabL from Bacillus subtilis. The Journal of biological chemistry. 2000 Dec 22; 275(51):40128-33. . PMID:11007778
Baldock C, Rafferty JB, Sedelnikova SE, Baker PJ, Stuitje AR, Slabas AR, Hawkes TR, Rice DW A mechanism of drug action revealed by structural studies of enoyl reductase. Science (New York, N.Y.). 1996 Dec 20; 274(5295):2107-10. . PMID:8953047

60A519E4C1485B9F5BF36E83EDE884B396857CB4

Page visits: 4650

Time of last update: 2025-04-04 12:00:51

Author of last update: Melvin.boenninger