fabF

fabF
168

beta-ketoacyl-acyl carrier protein synthase II, involved in the control of membrane fluidity

Locus
BSU_11340
Molecular weight
43.84 kDa
Isoelectric point
4.77
Protein length
413 aaSequenceBlast
Gene length
1242 bpSequenceBlast
Function
fatty acid biosynthesis
Product
beta-ketoacyl-acyl carrier protein synthase II
Essential
yes
E.C.
2.3.1.179
Synonyms
fabF, yjaY
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0304 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,209,183  1,210,424
Phenotypes of a mutant
essential PubMed
CRISPRi knock-down: terminal phenotype of growth cessation, moderate bending PubMed
FabF-I109F: resistance to cerulenin PubMed
FabF-L112F, FabF-I109M: resistance to cerulenin (Zeigler DR, unpublished observations)
The protein
Catalyzed reaction/ biological activity
(11Z)-hexadecenoyl-[ACP] + H+ + malonyl-[ACP] --> 3-oxo-(13Z)-octadecenoyl-[ACP] + CO2 + holo-[ACP](according to UniProt)
SigW-dependent expression of ''fabF and the yuaF-floT-yuaI'' operon result in reduced membrane fluidity PubMed
Protein family
thiolase-like superfamily (according to UniProt)
Structure
4LS5 (PDB) PubMed
O34340 (AlphaFold)
Expression and Regulation
Operons
Genes
fabHA-fabF
Description
PubMed
Regulation
''fabHA'': expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
Regulatory mechanism
FapR: repression, PubMed, in fapR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigW: sigma factor, PubMed, in sigW regulon
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fabHA→fabF

2025-04-05 08:25:15

ghost

111

f0300ca7f95f5d09f6fd443d20e6bb5dc275e224

89D91E1674F230E7B7EA16C855B9996B84C440D3

Biological materials
Mutant
BEC11340 (lacA::Pxyl-dcas9  amyE::Pveg-sgRNA(fabF) trpC2) BGSC, PubMed
1A573 (fabF2 trpC2) BGSC, cerulenin-resistant mutant
1A576 (fabF14 trpC2) BGSC, cerulenin-resistant mutant
1A577 (fabF20 trpC2) BGSC, cerulenin-resistant mutant
References
Reviews
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Original Publications
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5D5D9A544295DD9B55C75A8CF47AB19935E740C6

Page visits: 6316

Time of last update: 2025-04-16 15:27:24

Author of last update: Jstuelk