bioB

bioB
168

biotin synthase

Locus
BSU_30200
Molecular weight
36.77 kDa
Isoelectric point
5.55
Protein length
335 aaSequenceBlast
Gene length
1008 bpSequenceBlast
Function
biosynthesis of biotin
Product
biotin synthase
Essential
no
E.C.
2.8.1.6
Synonyms
bioB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0502 (Galperin et al., 2021)

This gene is a member of the following regulons

The protein
Catalyzed reaction/ biological activity
[sulfur carrier]-SH + dethiobiotin + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
Protein family
Radical SAM superfamily (according to UniProt)
Cofactors
Fe-S cluster PubMed
Structure
1R30 (PDB) (from E. coli, 36% identity) PubMed
P53557 (AlphaFold)
Expression and Regulation
Operons
Genes
bioW-bioA-bioF-bioD-bioB-bioI-ytbQ
Description
PubMed
Regulation
repressed in the presence of biotin (BirA) PubMed
Regulatory mechanism
BirA: repression, PubMed, in birA regulon
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bioW→ytbQ

2025-04-03 13:40:07

ghost

82

8e74711c48d33750c45114afa6e28dd08cdf4ea5

F7255B320407AE19B7B38F8E863B5EF4A9BF3D52

Biological materials
Mutant
GP4752 (trpC2 ΔbioB::neo), available in Jörg Stülke's lab
GP4764 (trpC2 ΔbioB::lox72), available in Jörg Stülke's lab
BKE30200 (bioB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAGTTCCATCCATTGATTCA,  downstream forward: _UP4_TGAAAGAATCAATAAAAGCA
BKK30200 (bioB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAGTTCCATCCATTGATTCA,  downstream forward: _UP4_TGAAAGAATCAATAAAAGCA
References
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5880617EB95FC0D19219A868D6D0A3D1D0D9C532

Page visits: 4003

Time of last update: 2025-04-14 01:34:53

Author of last update: Jstuelk