aroA

aroA
168

3-deoxy-D-arabino-heptulosonate 7-phosphate synthase / chorismate mutase-isozyme 3

Locus
BSU_29750
Molecular weight
39.38 kDa
Isoelectric point
5.34
Protein length
Gene length
Function
biosynthesis of aromatic amino acids
Product
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase /
Essential
no
E.C.
2.5.1.54
Synonyms
aroA, aroG

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2876 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,045,445 3,046,521
The protein
Catalyzed reaction/ biological activity
D-erythrose 4-phosphate + H2O + phosphoenolpyruvate --> 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate (according to UniProt)
chorismate --> prephenate (according to UniProt)
Protein family
class-I DAHP synthase family (single member, according to UniProt)
Chorismate mutase domain (aa 1-90) (according to UniProt)
Structure
1VR6 (PDB) (from Thermotoga maritima, 51% identity, 68% similarity)
5GO2 (PDB) (chorismate mutase-like domain, with citrate) PubMed
Modification
phosphorylation on Ser-2 PubMed
phosphorylation on Thr-4 PubMed
phosphorylated on Arg-45 and Arg-301 PubMed
Cys126 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed
Effectors of protein activity
subject to feedback inhibition PubMed
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
Description
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aroA

2025-03-01 22:00:11

ghost

85

87ef7376a3fd4442d768a3a2fbe85729e8570134

E34FF0014B03F671CED03422AD84D4FD91310A77

Biological materials
Mutant
BKE29750 (aroA::erm trpC2) available at BGSC and in Jrg Stlke's lab, PubMed, upstream reverse: _UP1_CATTTTTTTCATCCTTTCCC, downstream forward: _UP4_TAATTGAACAATCCAAAAGG
BKK29750 (aroA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTTCATCCTTTCCC, downstream forward: _UP4_TAATTGAACAATCCAAAAGG
References
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75E331ABCDE1C60AAB4AC01229CED81B0AF50E1C

Page visits: 7119

Time of last update: 2025-04-05 16:16:28

Author of last update: Jstuelk