bshB2

bshB2
168

N-acetylglucosamine-malate deacetylase, minor enzyme involved in bacillithiol synthesis, may act as amidase for the processing of BSH conjugates

Locus
BSU_19460
Molecular weight
16.54 kDa
Isoelectric point
4.51
Protein length
221 aaSequenceBlast
Gene length
666 bpSequenceBlast
Function
biosynthesis of bacillithiol
Product
N-acetylglucosamin-malate deacetylase
Essential
no
Synonyms
bshB2, yojG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2120 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,121,641  2,122,306
The protein
Catalyzed reaction/ biological activity
(S)-malyl N-acetyl-α-D-glucosaminide + H2O --> (S)-malyl α-D-glucosaminide + acetate (according to UniProt)
Protein family
PIGL family (with BshB1, according to UniProt)
Structure
O31857 (AlphaFold)
Paralogous protein(s)
BshB1
Expression and Regulation
Operons
Genes
yoyC-yojF-bshB2
Description
(major transcript) PubMed
Regulation
''yoyC'': induced by diamide stess (thiol depletion) (Spx) PubMed
Regulatory mechanism
Spx: activation, PubMed, in spx regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

yoyC→bshB2

2025-03-17 18:39:53

ghost

115

d45be8e59e1ef5518f8145b4813c89824dd3662c

590D15116157EC6EF159535B7BEBBAA02AA514C8

Genes
yojE-yoyC-yojF-bshB2
Description
PubMed
Regulation
''yoyC'': induced by diamide stess (thiol depletion) (Spx) PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

yojE→bshB2

2025-03-17 19:16:51

ghost

88

8005c717823da3d26388a7c7eb8015403326f861

6896F6A609EE8E15A33B13727D3C844A671832BA

Biological materials
Mutant
MGNA-B428 (yojG::erm), available at the NBRP B. subtilis, Japan
bshB2 null mutant and ''bshB1 / bshB2'' double null mutant available in John Helmann lab
CS212 (''bshB2::aphA3'', available in Colin Harwood's and Jörg Stülke's labs) PubMed
BKE19460 (bshB2::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCATTATTATTCACCCTT,  downstream forward: _UP4_TAATCAAAAAGGTATAAGGC
BKK19460 (bshB2::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCATTATTATTCACCCTT,  downstream forward: _UP4_TAATCAAAAAGGTATAAGGC
References
Reviews
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Original Publications
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8106404D9D2CFA0825E1284D47CC7198D8A20E5D

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Time of last update: 2025-04-17 08:07:45

Author of last update: Jstuelk