yrrN

yrrN
168

tRNA uridine hydroxylase (in complex with YrrO)

Locus
BSU_27350
Molecular weight
34.92 kDa
Isoelectric point
4.64
Protein length
309 aaSequenceBlast
Gene length
930 bpSequenceBlast
Function
introduction of 5-methoxyuridine modification in tRNA (U34)
Product
tRNA uridine hydroxylase
Essential
no
E.C.
null
Synonyms
yrrN, trhP1
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0826 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,794,147  2,795,076
Phenotypes of a mutant
50% reduction in mo5U tRNA levels PubMed
The protein
Catalyzed reaction/ biological activity
hydroxylation of U34 in tRNA to give 5-hydroxyuridine (ho5U) (in complex with YrrO) with prephenate as hydroxyl donor, ho5U is the precursor for 5-methoxyuridine modification (by TrmR)  PubMed
Protein family
peptidase U32 family (with YrrO, according to UniProt)
Structure
O32035 (AlphaFold)
Paralogous protein(s)
YrrO
Expression and Regulation
Operons
Genes
trmR-yrrN-yrrO-udk-greA
Description
PubMed
Open in new tab

trmR→greA

2025-03-17 19:57:18

Jstuelk

178

0a66c040f2f5a1d47c0559c4047af2666d30c835

5807E821D8403F7EC565573C8910C732811F8BB4

Biological materials
Mutant
MGNA-A008 (yrrN::erm), available at the NBRP B. subtilis, Japan
BKE27350 (yrrN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACGTTCACCTCTTCTT,  downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
BKK27350 (yrrN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACGTTCACCTCTTCTT,  downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
References
Research papers
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A3EC327DA14F188E88AF4221B51EE5950F882277

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Time of last update: 2025-04-06 17:33:16

Author of last update: Jstuelk