darB

darB
168

c-di-AMP binding protein, stimulates (p)ppGpp accumulation under conditions of potassium starvation

Locus
BSU_14130
Molecular weight
16.49 kDa
Isoelectric point
5.16
Protein length
147 aaSequenceBlast
Gene length
444 bpSequenceBlast
Function
control of Rel activity, trigger of the stringent response under conditions of potassium starvation
Product
c-di-AMP binding protein
Essential
no
Synonyms
darB, ykuL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
1,485,453  1,485,896
Phenotypes of a mutant
inactivation of darB facilitates the adaptation of a strain lacking c-di-AMP to growth on complex medium PubMed
The protein
Catalyzed reaction/ biological activity
apo-DarB (under conditions of potassium starvation) binds to Rel to trigger (p)ppGpp accumulation PubMed
Domains
CBS domain (aa 18-78) (according to UniProt)
Structure
6YJ8 (PDB) (Apo-DarB) PubMed
6YJA (PDB) (complex with c-di-AMP) PubMed
8ACU (PDB) (the DarB2-RelNTD2 complex) PubMed
O31698 (AlphaFold)
Effectors of protein activity
binds c-di-AMP PubMed, this prevents binding to Rel PubMed
Expression and Regulation
Operons
Genes
ykuJ-ykuK-abbA-darB-ccpC
Description
PubMed
Regulation
expressed under conditions that trigger sporulation (Spo0A) PubMed
Regulatory mechanism
Spo0A: activation, by Spo0A~P PubMed, in spo0A regulon
CcpA: repression, PubMed, in ccpA regulon
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ykuJ→ccpC

2025-03-21 21:09:48

ghost

156

c31a71bd4220fdbe189c19124eb835899db4473b

DE202F49BDBFA88FBD49A4685EB1CB8595CFCE08

Biological materials
Mutant
MGNA-A773 (darB::erm), available at the NBRP B. subtilis, Japan
GP2769 (ΔdarB::erm) available in Jörg Stülke's lab
GP3409(ΔdarB::cat) available in Jörg Stülke's lab
BKE14130 (darB::erm  trpC2) available in the Bacillus Genetic Stock Center and in Jörg Stülke's lab PubMed
GP214 (deletion of ykuJ-ykuK-abbA-darB, replaced by kan), available in Jörg Stülke's lab
BKE14130 (darB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTCGACCCCTTTTC,  downstream forward: _UP4_TAGGCTGTACGGTCCTATTT
BKK14130 (darB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTCGACCCCTTTTC,  downstream forward: _UP4_TAGGCTGTACGGTCCTATTT
GP2800 (ΔdarB-ccpC::phleo), available in Jörg Stülke's lab
Expression vectors
pGP2929 (N-terminal 6xHis-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP2972 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
pGP635: expression of Strep-darB by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
pGP767: expression of darB-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
pGP3306: expression of darB by pBQ200 in B. subtilis, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP2810 darB-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
References
Reviews
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Original Publications
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90AFF93D6E7BC993B2773C12EE400C80A87A4831

Page visits: 6627

Time of last update: 2025-04-03 19:45:56

Author of last update: Dennis.wicke