yfmL

yfmL
168

DEAD-box RNA helicase

Locus
BSU_07430
Molecular weight
42.03 kDa
Isoelectric point
9.6
Protein length
376 aaSequenceBlast
Gene length
1131 bpSequenceBlast
Function
RNA helicase
Product
DEAD-box RNA helicase
Essential
no
Synonyms
yfmL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0513 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
816,113  817,243
Phenotypes of a mutant
poor growth at low temperatures (16 to 20C) PubMed
The protein
Catalyzed reaction/ biological activity
RNA helicase
required for ribosome assembly PubMed
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
helicase family (according to UniProt)
DEAD-box RNA helicases (according to UniProt)
Domains
Helicase ATP-binding domain (aa 35-205) (according to UniProt)
Helicase C-terminal domain (aa 231-374) (according to UniProt)
Structure
5IVL (PDB) (CshA, 87% coverage, 35% identity) PubMed
O34750 (AlphaFold)
Paralogous protein(s)
CshB, CshA, DeaD
Expression and Regulation
Operons
Genes
yfmL
Description
PubMed
Regulation
expression declines in the stationary phase PubMed
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yfmL

2025-04-03 03:45:54

Jstuelk

54

196ae6d1f911e8cb9511675864d798bf201444ca

B4271C5451E1D7D81F44805A6ACF01D03DD803BA

Biological materials
Mutant
MGNA-C239 (yfmL::erm), available at the NBRP B. subtilis, Japan
GP1053 (yfmL::erm), available in the Jörg Stülke's lab PubMed
BKE07430 (yfmL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTGTTCTCCTCAATTC,  downstream forward: _UP4_TAAAAAGAAGGGCGAAGAGC
BKK07430 (yfmL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTGTTCTCCTCAATTC,  downstream forward: _UP4_TAAAAAGAAGGGCGAAGAGC
Expression vectors
for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1069 (spc), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed
FLAG-tag construct
GP1066 (spc, based on pGP1331), available in Jörg Stülke's lab
GFP fusion
B. subtilis GP1082 yfmL-yfp spc, available in Jörg Stülke's lab
References
Reviews
Hussain ADEAD Box RNA Helicases: Biochemical Properties, Role in RNA Processing and Ribosome Biogenesis.Cell biochemistry and biophysics. 2024 Mar 2; . PMID: 38430409
Original Publications
González-Gutiérrez JA, Díaz-Jiménez DF, Vargas-Pérez I, Guillén-Solís G, Stülke J, Olmedo-Álvarez G The DEAD-Box RNA Helicases of as a Model to Evaluate Genetic Compensation Among Duplicate Genes. Frontiers in microbiology. 2018; 9:2261. doi:10.3389/fmicb.2018.02261. PMID:30337909
Huen J, Lin CL, Golzarroshan B, Yi WL, Yang WZ, Yuan HS Structural Insights into a Unique Dimeric DEAD-Box Helicase CshA that Promotes RNA Decay. Structure (London, England : 1993). 2017 Mar 07; 25(3):469-481. pii:S0969-2126(17)30012-6. doi:10.1016/j.str.2017.01.012. PMID:28238534
Lehnik-Habrink M, Rempeters L, Kovács ÁT, Wrede C, Baierlein C, Krebber H, Kuipers OP, Stülke J DEAD-Box RNA helicases in Bacillus subtilis have multiple functions and act independently from each other. Journal of bacteriology. 2013 Feb; 195(3):534-44. doi:10.1128/JB.01475-12. PMID:23175651

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Time of last update: 2025-04-06 22:17:50

Author of last update: Jstuelk