thyB

thyB
168

thymidylate synthase B

Locus
BSU_21820
Molecular weight
30.39 kDa
Isoelectric point
6.27
Protein length
264 aaSequenceBlast
Gene length
795 bpSequenceBlast
Function
biosynthesis of thymidine nucleotides
Product
thymidylate synthase B
Essential
no
E.C.
2.1.1.45
Synonyms
thyB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0207 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,297,106  2,297,900
The protein
Catalyzed reaction/ biological activity
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP --> 7,8-dihydrofolate + dTMP (according to UniProt)
Protein family
thymidylate synthase family (with ThyA, according to UniProt)
Structure
3IX6 (PDB) (from Brucella melitensis, 67% identity)
P11044 (AlphaFold)
Modification
phosphorylated on Arg-102 PubMed
Paralogous protein(s)
ThyA
Expression and Regulation
Operons
Genes
ypjP-ypjQ-thyB-dfrA-ypkP
Description
PubMed
Open in new tab

ypjP→ypkP

2025-04-04 00:36:43

Jstuelk

109

235b0d972d347f04b87f4c45ebac71a8a9d4b96a

9EB04D4A3A7698B00A36018A9A11CB02D30C8250

Genes
thyB-dfrA
Description
PubMed
Regulation
weak induction in the presence of glucose (CcpN) PubMed
Regulatory mechanism
CcpN: activation, weak, in ccpN regulon
Open in new tab

thyB→dfrA

2025-04-04 22:01:17

ghost

81

c85a29ba6a879af8f10ee961ab515c0e0a8b2658

B9BDE226BFA3A6A21A7E3540C80A424429DFA9B3

Biological materials
Mutant
BKE21820 (thyB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTTCATCCTTTAAA,  downstream forward: _UP4_GTATGATTTCATTCATTTTT
BKK21820 (thyB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTTCATCCTTTAAA,  downstream forward: _UP4_GTATGATTTCATTCATTTTT
References
Reviews
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Original Publications
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9F9643D51E9ABA537892B19312491697CECDDCCF

Page visits: 3191

Time of last update: 2025-04-04 02:40:35

Author of last update: Melvin.boenninger