gamA

gamA
168

glucosamine-6-phosphate deaminase

Locus
BSU_02360
Molecular weight
27.14 kDa
Isoelectric point
5.79
Protein length
249 aaSequenceBlast
Gene length
750 bpSequenceBlast
Function
glucosamine utilization
Product
glucosamine-6-phosphate deaminase
Essential
no
E.C.
null
Synonyms
gamA, ybfT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0363 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
256,823  257,572
Phenotypes of a mutant
no growth on glucosamine PubMed
The protein
Catalyzed reaction/ biological activity
α-D-glucosamine 6-phosphate + H2O --> β-D-fructose 6-phosphate + NH4+ (according to UniProt)
Protein family
glucosamine/galactosamine-6-phosphate isomerase family (with NagB, according to UniProt)
Structure
2BKX (PDB) (the structure of NagB, 50% identity) PubMed
O31458 (AlphaFold)
Kinetic information
K(M): 3.0 mM PubMed
Paralogous protein(s)
NagB
Expression and Regulation
Operons
Genes
gamA-gamP
Description
PubMed
Regulation
induced by glucosamine (GamR) PubMed
Regulatory mechanism
GamR: repression, PubMed, in gamR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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gamA→gamP

2025-03-09 02:38:53

ghost

66

4fffcf05befb972653171bbbe342dd9b2c49f8b3

1347DE6686FBBF7ED2CBD5C47BA6DFFC67338C93

Biological materials
Mutant
MGNA-B938 (ybfT::erm), available at the NBRP B. subtilis, Japan
BKE02360 (gamA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGACACCCCCTCAAAG,  downstream forward: _UP4_TAACACAATAAAAGGAGACT
BKK02360 (gamA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGACACCCCCTCAAAG,  downstream forward: _UP4_TAACACAATAAAAGGAGACT
References
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8BBC9AFF4CD56A3A66E270A2BBA193D095EE01D1

Page visits: 4803

Time of last update: 2025-04-05 19:25:19

Author of last update: Melvin.boenninger