abfA

abfA
168

alpha-L-arabinofuranosidase

Locus
BSU_28720
Molecular weight
56.89 kDa
Isoelectric point
5.34
Protein length
500 aaSequenceBlast
Gene length
1503 bpSequenceBlast
Function
arabinan degradation
Product
alpha-L-arabinofuranosidase
Essential
no
E.C.
3.2.1.55
Synonyms
abfA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3534 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,938,330  2,939,832
The protein
Catalyzed reaction/ biological activity
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides (according to UniProt)
Protein family
glycosyl hydrolase 51 family (with Abf2, according to UniProt)
Structure
1QW8 (PDB) (complex with Ara-alpha-Xyl, Geobacillus stearothermophilus),  1PZ3 (PDB) (Geobacillus stearothermophilus)
P94531 (AlphaFold)
Localization
forms hexamers PubMed
Expression and Regulation
Operons
Genes
araA-araB-araD-araL-araM-araN-araP-araQ-abfA
Description
PubMed
Regulation
induced by arabinose (AraR) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
AraR: repression, PubMed, in araR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
the mRNA is very stable (half-life > 15 min) PubMed
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araA→abfA

2025-04-03 18:38:59

ghost

145

b7d3312ef868c449eb18db3a2b2bf36130567026

7BE2C086CD88758AEADFA2166B6FC6182EB55613

Biological materials
Mutant
BKE28720 (abfA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCATCACACGTTTCCTCC,  downstream forward: _UP4_TAAGAATAGCAAAGCCGGAG
BKK28720 (abfA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCATCACACGTTTCCTCC,  downstream forward: _UP4_TAAGAATAGCAAAGCCGGAG
References
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2B2629D275491A9F7031D5A99BAA001B011A765B

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Time of last update: 2025-04-13 20:56:34

Author of last update: Jstuelk