yycR

yycR
168

formaldehyde dehydrogenase

Locus
BSU_40250
Molecular weight
42.79 kDa
Isoelectric point
5.79
Protein length
408 aaSequenceBlast
Gene length
1227 bpSequenceBlast
Function
detoxification of formaldehyde
Product
formaldehyde dehydrogenase
Essential
no
Synonyms
yycR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1063 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,135,798  4,137,024
Phenotypes of a mutant
reduced tolerance to formaldehyde PubMed
The protein
Catalyzed reaction/ biological activity
formaldehyde + H2O + NAD+ --> formate + 2 H+ + NADH PubMed
Protein family
zinc-containing alcohol dehydrogenase family (according to UniProt)
Cofactors
NAD+ PubMed
Zn(2+) PubMed
Structure
4JLW (PDB) (from Pseudomonas aeruginosa, 66% identity) PubMed
Q45604 (AlphaFold)
Kinetic information
Km: 0.19 ± 0.05 mM PubMed
Vmax: 2.24 ± 0.05 nmol min−1 PubMed
Paralogous protein(s)
AdhB, (33%),alcohol dehydrogenase
Expression and Regulation
Operons
Genes
yycR
Description
PubMed
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yycR

2025-02-23 16:42:26

Jstuelk

48

5826660f5e7eecd79bdab32bc77079667831120a

2E4886C575FAA10B8F4F0CC11669F55B43D88BC2

Biological materials
Mutant
MGNA-B816 (yycR::erm), available at the NBRP B. subtilis, Japan
BKE40250 (yycR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATGTGTTTGGACTCT,  downstream forward: _UP4_TAAAAGAAAAAGCCGGACGT
BKK40250 (yycR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATGTGTTTGGACTCT,  downstream forward: _UP4_TAAAAGAAAAAGCCGGACGT
References
Research papers
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Page visits: 2006

Time of last update: 2025-04-03 11:25:46

Author of last update: Jstuelk