yxeK

yxeK
168

N-acetyl S-(2-succino)cysteine oxygenase, detoxification of S-(2-succino)-adducts via oxygenolytic C-S bond cleavage

Locus
BSU_39520
Molecular weight
49.18 kDa
Isoelectric point
5.67
Protein length
441 aaSequenceBlast
Gene length
1326 bpSequenceBlast
Function
utilization and detoxification of S-(2-succino)cysteine
Product
N-acetyl S-(2-succino)cysteine oxygenase
Essential
no
Synonyms
yxeK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG2141 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CymR regulon

Gene
Coordinates
4,060,818  4,062,143
Phenotypes of a mutant
no growth with S-(2-succino)cysteine as the single source of sulfur PubMed
The protein
Catalyzed reaction/ biological activity
oxygenation of N-acetyl S-(2-succino)cysteine, resulting in C-S bond cleavage (N-acetylcysteine + oxaloacetate) PubMed
Protein family
ntaA/snaA/soxA(dszA) monooxygenase family (with CmoJ, according to UniProt)
Cofactors
FMN PubMed
Structure
5TLC (PDB) (from B. subtilis WU-S2B 42% identity) PubMed
P54950 (AlphaFold)
Paralogous protein(s)
CmoJ
Expression and Regulation
Operons
Genes
yxeK-snaB-yxeM-yxeN-yxeO-sndB-yxeQ
Description
PubMed
Regulation
repressed in the presence of cysteine (CymR) PubMed
Regulatory mechanism
CymR: repression, PubMed, in cymR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed
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yxeK→yxeQ

2025-03-31 02:55:29

Bzhu

101

26e7d8a77b7f64e210508bce49d61eed26cdd5aa

11F447FD5833B59A1639842D70685F9A6B369EAF

Biological materials
Mutant
MGNA-B709 (yxeK::erm), available at the NBRP B. subtilis, Japan
BKE39520 (yxeK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCGATACTCCTTTTAT,  downstream forward: _UP4_TATGCAAAGTAAGGAGGAAC
BKK39520 (yxeK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCGATACTCCTTTTAT,  downstream forward: _UP4_TATGCAAAGTAAGGAGGAAC
References
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19E6EF9CCA31440EAC046F8D6CA98B6A08589D58

Page visits: 4562

Time of last update: 2025-04-05 19:22:10

Author of last update: Jstuelk