trmR

trmR
168

tRNA wobble uridine modifying methyltransferase

Locus
BSU_27360
Molecular weight
24.92 kDa
Isoelectric point
5.57
Protein length
217 aaSequenceBlast
Gene length
654 bpSequenceBlast
Function
introduction of 5-methoxyuridine modification in tRNA (U34)
Product
tRNA wobble uridine modifying methyltransferase
Essential
no
Synonyms
trmR, yrrM
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG4122 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,795,082 → 2,795,735
The protein
Catalyzed reaction/ biological activity
methylation of (5-hydroxyuridine) ho5U containing tRNA to 5-methoxyuridine (mo5U) PubMed
5-hydroxyuridine34 in tRNA + S-adenosyl-L-methionine --> 5-methoxyuridine34 in tRNA + H+ + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
Methyltransferase superfamily (according to UniProt)
class I-like SAM-binding methyltransferase superfamily (according to UniProt)
Cofactors
SAM PubMed
Structure
5ZW3 (PDB) PubMed
5ZW4 (PDB) (bound to tRNA) PubMed
O32036 (AlphaFold)
Expression and Regulation
Operons
Genes
trmR-yrrN-yrrO-udk-greA
Description
PubMed
Open in new tab

trmR→greA

2025-03-17 19:57:18

Jstuelk

178

0a66c040f2f5a1d47c0559c4047af2666d30c835

5807E821D8403F7EC565573C8910C732811F8BB4

Biological materials
Mutant
MGNA-A853 (yrrM::erm), available at the NBRP B. subtilis, Japan
BKE27360 (ΔtrmR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTACAAACAGCCTCCCGT,  downstream forward: _UP4_AGTAAAAAGAAGAGGTGAAC
BKK27360 (ΔtrmR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTACAAACAGCCTCCCGT,  downstream forward: _UP4_AGTAAAAAGAAGAGGTGAAC
References
Research papers
Loading

CE4F2965077F2A5882432D683520E09B70B9369A

Page visits: 2359

Time of last update: 2025-04-06 17:30:52

Author of last update: Melvin.boenninger