YitJ

• Description: methionine synthase
  
  

Gene name	yitJ
Synonyms
Essential	no
Product	methionine synthase
Function	methionine biosynthesis
Gene expression levels in SubtiExpress: yitJ
Metabolic function and regulation of this protein in SubtiPathways: yitJ
MW, pI	67 kDa, 5.336
Gene length, protein length	1836 bp, 612 aa
Immediate neighbours	yitI, yitK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU11010

Database entries

• BsubCyc: BSU11010

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane associated PubMed

Database entries

• BsubCyc: BSU11010

• Structure:

• UniProt: O06745

• KEGG entry: [2]

• E.C. number: 2.1.1.13

Expression and regulation

• Operon: yitJ-yitI-yitH PubMed

• Expression browser: yitJ PubMed

• Sigma factor:

• Regulation:
  • induced by methionine starvation (S-box) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 894 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

The S-box

Vamsi Krishna Boyapati, Wei Huang, Jessica Spedale, Fareed Aboul-Ela
Basis for ligand discrimination between ON and OFF state riboswitch conformations: the case of the SAM-I riboswitch.
RNA: 2012, 18(6);1230-43
[PubMed:22543867] [WorldCat.org] [DOI] (I p)

Changrui Lu, Fang Ding, Anirban Chowdhury, Vineeta Pradhan, Jerneja Tomsic, W Michael Holmes, Tina M Henkin, Ailong Ke
SAM recognition and conformational switching mechanism in the Bacillus subtilis yitJ S box/SAM-I riboswitch.
J Mol Biol: 2010, 404(5);803-18
[PubMed:20951706] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Jerneja Tomsic, Brooke A McDaniel, Frank J Grundy, Tina M Henkin
Natural variability in S-adenosylmethionine (SAM)-dependent riboswitches: S-box elements in bacillus subtilis exhibit differential sensitivity to SAM In vivo and in vitro.
J Bacteriol: 2008, 190(3);823-33
[PubMed:18039762] [WorldCat.org] [DOI] (I p)

F J Grundy, T M Henkin
The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.
Mol Microbiol: 1998, 30(4);737-49
[PubMed:10094622] [WorldCat.org] [DOI] (P p)