MtlD

• Description: mannitol-1-phosphate 5-dehydrogenase
  
  

Gene name	mtlD
Synonyms	mtlB
Essential	no
Product	mannitol-1-phosphate 5-dehydrogenase
Function	mannitol utilization
Gene expression levels in SubtiExpress: mtlD
Metabolic function and regulation of this protein in SubtiPathways: mtlD
MW, pI	40 kDa, 6.291
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	mtlF, ycsA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

MtlR regulon

The gene

Basic information

• Locus tag: BSU03990

Database entries

• BsubCyc: BSU03990

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-mannitol 1-phosphate + NAD⁺ = D-fructose 6-phosphate + NADH (according to Swiss-Prot)

• Protein family: mannitol dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU03990

• Structure: 3H2Z (from Shigella flexneri 2a str. 2457t mutant, 42% identity, 59% similarity)

• UniProt: P42957

• KEGG entry: [3]

• E.C. number: 1.1.1.17

Expression and regulation

• Operon: mtlA-mtlF-mtlD PubMed

• Expression browser: mtlD PubMed

• Sigma factor:

• Regulation:
  • induced by mannitol (MtlR) PubMed
  • repressed by glucose (MtlR) PubMed

• Regulatory mechanism:
  • MtlR: transcription activation PubMed

• Additional information:
  • An antisense RNA is predicted for mtlD PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 32 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: