Difference between revisions of "RbgA"
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* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 94 {{PubMed|24696501}} | ||
+ | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 568 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = |
Revision as of 09:52, 17 April 2014
- Description: assembly of the 50S subunit of the ribosome
Gene name | rbgA |
Synonyms | ylqF |
Essential | yes PubMed |
Product | GTPase |
Function | ribosome assembly |
Gene expression levels in SubtiExpress: rbgA | |
MW, pI | 31 kDa, 9.528 |
Gene length, protein length | 846 bp, 282 aa |
Immediate neighbours | rplS, rnhB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
translation, essential genes, GTP-binding proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16050
Phenotypes of a mutant
- essential PubMed
- depletion confers a slow growth phenotype, and induces an abnormality of the in vivo ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes PubMed
- premature 45S particles accumulated in RbgA-depleted cells lack several proteins, including L16, L27, L28, L33, L35 and L36 PubMed
Database entries
- BsubCyc: BSU16050
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
- Protein family: MTG1 subfamily (according to Swiss-Prot) Era/Obg family
Extended information on the protein
- Kinetic information:
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU16050
- Structure: 1PUJ
- UniProt: O31743
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
Your additional remarks
References
Reviews
Original publications