bioD

bioD
168

dethiobiotin synthase

Locus
BSU_30210
Molecular weight
25.01 kDa
Isoelectric point
6.21
Protein length
231 aaSequenceBlast
Gene length
696 bpSequenceBlast
Function
biosynthesis of biotin
Product
dethiobiotin synthase
Essential
no
E.C.
6.3.3.3
Synonyms
bioD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0132 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,091,492 → 3,092,187
The protein
Catalyzed reaction/ biological activity
7,8-diaminononanoate + ATP + CO2 = ADP + dethiobiotin + 3 H+ + phosphate (according to UniProt)
Protein family
dethiobiotin synthetase family (single member, according to UniProt)
Structure
1DTS (PDB) (from E. coli, 32% identity) PubMed
P53558 (AlphaFold)
Expression and Regulation
Operons
Genes
bioW-bioA-bioF-bioD-bioB-bioI-ytbQ
Description
PubMed
Regulation
repressed in the presence of biotin (BirA) PubMed
Regulatory mechanism
BirA: repression, PubMed, in birA regulon
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bioW→ytbQ

2025-04-03 13:40:07

ghost

82

8e74711c48d33750c45114afa6e28dd08cdf4ea5

F7255B320407AE19B7B38F8E863B5EF4A9BF3D52

Biological materials
Mutant
GP4751 (trpC2 ΔbioD::neo), available in Jörg Stülke's lab
BKE30210 (ΔbioD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGTTCCCGTCACAAAAAAAC,  downstream forward: _UP4_ATGAATCAAGTGGGGGTATG
BKK30210 (ΔbioD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGTTCCCGTCACAAAAAAAC,  downstream forward: _UP4_ATGAATCAAGTGGGGGTATG
References
Reviews
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Original Publications
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D9A71DAF949FB6C9C22C8361D83C5A0741184977

Page visits: 5762

Time of last update: 2025-04-13 19:16:56

Author of last update: Robert.warneke