PrfA

• Description: peptide chain release factor 1
  
  

Gene name	prfA
Synonyms
Essential	yes PubMed
Product	peptide chain release factor 1
Function	translation
Gene expression levels in SubtiExpress: prfA
MW, pI	40 kDa, 4.824
Gene length, protein length	1068 bp, 356 aa
Immediate neighbours	ywkE, ywkD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

The gene

Basic information

• Locus tag: BSU37010

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU37010

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfA is highly specific for a U in the first stop-codon position and recognizes UAA and UAG

• Protein family: prokaryotic/mitochondrial release factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU37010

• Structure:

• UniProt: P45872

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: prfA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 541 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1958 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Johan Sund, Martin Andér, Johan Aqvist
Principles of stop-codon reading on the ribosome.
Nature: 2010, 465(7300);947-50
[PubMed:20512119] [WorldCat.org] [DOI] (I p)

Daniel J Rigden, Peter Setlow, Barbara Setlow, Irina Bagyan, Richard A Stein, Mark J Jedrzejas
PrfA protein of Bacillus species: prediction and demonstration of endonuclease activity on DNA.
Protein Sci: 2002, 11(10);2370-81
[PubMed:12237459] [WorldCat.org] [DOI] (P p)