infB

infB
168

translation initiation factor IF-2, ribosome-dependent GTPase interacting with 30 ribosome subunits, ensures correct binding of initiator tRNAs

Locus
BSU_16630
Molecular weight
78.35 kDa
Isoelectric point
5.19
Protein length
Gene length
Function
translation
Product
initiation factor IF-2
Essential
yes
Synonyms
infB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0532 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,734,009  1,736,159
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
ribosome-dependent GTPase interacting with 30 ribosome subunits, ensures correct binding of initiator tRNAs PubMed
Putative cellular metabolic sensor and regulator that oscillates between an active GTP-bound form under conditions allowing active protein syntheses and an inactive ppGpp-bound form when depleting of nutrients PubMed
Protein family
Nucleotide-binding domain that competitively binds GTP or ppGpp PubMed
tr-type G domain (aa 217-386) (according to UniProt)
Structure
1D1N (PDB) (fMet-tRNA-fMet binding domain, Geobacillus stearothermophilus),  1Z9B (PDB) (C1-subdomain, Geobacillus stearothermophilus)
Effectors of protein activity
GTP and ppGpp PubMed
cytoplasm (according to UniProt)
Expression and Regulation
Operons
Description
Regulation
autoregulation NusA PubMed
induced by glucose PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
NusA: attenuation, NusA stimulates termination PubMed, in nusA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigB: sigma factor, PubMed, in sigB regulon
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ylxSrbfA

2025-04-05 23:43:43

Jstuelk

129

019B939D583142A5395482C3EECB5B469F354080

A30223501809D45C115085980399F87C5C5A0442

References
Reviews
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Original Publications
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378B77412D0DEA220A97DEC246410509D9B74D46

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Time of last update: 2025-04-06 14:20:07

Author of last update: Jstuelk