PdxS

• Description: pyridoxal-5'-phosphate synthase (synthase domain)
  
  

Gene name	pdxS
Synonyms	yaaD
Essential	no
Product	pyridoxal-5'-phosphate synthase (synthase domain)
Function	pyridoxal-5'-phosphate biosynthesis
Gene expression levels in SubtiExpress: pdxS
Interactions involving this protein in SubtInteract: PdxS
Metabolic function and regulation of this protein in SubtiPathways: PdxS
MW, pI	31 kDa, 5.085
Gene length, protein length	882 bp, 294 aa
Immediate neighbours	dacA, pdxT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU00110

Phenotypes of a mutant

auxotrophic for pyridoxal 5'-phosphate PubMed

Database entries

• BsubCyc: BSU00110

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: pdxS/SNZ family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-8 PubMed
  • phosphorylated on STY PubMed, PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • PdxS-PdxT PubMed1 PubMed2

• Localization:

Database entries

• BsubCyc: BSU00110

• Structure:
  • 2NV1 PdxS PubMed
  • 2NV2 PdxS-PdxT complex PubMed

• UniProt: P37527

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pdxS-pdxT PubMed

• Expression browser: pdxS PubMed

• Sigma factor:

• Regulation: negatively controlled by Spo0A PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 6001 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 16290 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: