argG

argG
168

argininosuccinate synthase, reversible

Locus
BSU_29450
Molecular weight
44.66 kDa
Isoelectric point
5.03
Protein length
403 aaSequenceBlast
Gene length
1212 bpSequenceBlast
Function
biosynthesis of arginine
Product
argininosuccinate synthase, reversible
Essential
no
E.C.
6.3.4.5
Synonyms
argG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0137 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,013,133 3,014,344
The protein
Catalyzed reaction/ biological activity
ATP + L-aspartate + L-citrulline --> 2-(N-L-arginino)succinate + AMP + diphosphate + H+ (according to UniProt)
Protein family
argininosuccinate synthase family (single member, according to UniProt)
Structure
1KH1 (PDB) (from Thermus thermophilus, 44% identity, 65% similarity) PubMed
O34347 (AlphaFold)
Modification
S-cysteinylation after diamide stress (C187) PubMed
phosphorylated on Arg-262 PubMed
Localization
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
argG-argH
Description
PubMed
Regulation
repressed by arginine (AhrC)
induced in the absence of RnpM PubMed
Regulatory mechanism
AhrC: repression, in ahrC regulon
RnpM: unknown, PubMed, in rnpM regulon
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argG→argH

2025-03-05 01:06:45

Jstuelk

107

1c207e814f33fb3d04dcc49c4c9da5b8d9da0a69

F891D79BBED99BE9F3E6174F94CE2EF8EDC02509

Biological materials
Mutant
GP1688 (argG-argH)::aphA3, available in Jörg Stülke's lab
GP3711 (argG::aphA3 trpC2) available in the Stülke lab
GP3984 (amyE::PargGH-lacZ-aphA3) suitable for quantification of expression of the argGH operon, available in Jörg Stülke's lab
BKE29450 (argG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAAAAATCCCCTCTC, downstream forward: _UP4_GCATGAAGAAGCTTTGGGGA
BKK29450 (argG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAAAAATCCCCTCTC, downstream forward: _UP4_GCATGAAGAAGCTTTGGGGA
Expression vectors
pGP3766: expression of Strep-argG by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
pGP3778: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
LacZ fusion
GP3984 (aphA3, based on pAC7]), available in Jörg Stülke's lab
References
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Page visits: 5820

Time of last update: 2025-04-10 17:26:49

Author of last update: Robert.warneke