lexA

lexA
168

transcriptional repressor of the SOS regulon

Locus
BSU_17850
Molecular weight
22.70 kDa
Isoelectric point
5.16
Protein length
205 aaSequenceBlast
Gene length
618 bpSequenceBlast
Function
regulation of DNA damage repair
Product
transcriptional repressor of the SOS regulon
Essential
no
Synonyms
lexA, dinR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1974 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,917,639 → 1,918,256
The protein
Catalyzed reaction/ biological activity
Represses ''uvrB, bstG, tagC, recA'' genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair (according to UniProt)
Hydrolysis of Ala-|-Gly bond in repressor LexA (according to UniProt)
Protein family
peptidase S24 family (single member, according to UniProt)
Structure
1JHH (PDB) (from E. coli, S119A mutant, 33% identity, 52% similarity) PubMed
P31080 (AlphaFold)
Expression and Regulation
Operons
Genes
lexA
Description
PubMed
Regulation
induced upon DNA damage (LexA) PubMed
Regulatory mechanism
LexA: repression, PubMed, in lexA regulon
RocR: autorepression, in rocR regulon
Additional information
A ncRNA is predicted between 'fosB' and 'lexA' PubMed
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lexA

2025-03-18 22:51:19

ghost

105

50063d2a47ceff152540c175cf10ffe58a62eb8b

AF4E25AB87B1EDC97FEE373493E80437751689F7

Biological materials
Mutant
BKE17850 (ΔlexA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTCGCACCTCAAAAC,  downstream forward: _UP4_TAATCTATTGATCTATCAGA
BKK17850 (ΔlexA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTCGCACCTCAAAAC,  downstream forward: _UP4_TAATCTATTGATCTATCAGA
References
Reviews
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Original Publications
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D267AF38B0CF107042326E9B8F3DD3C9C85840E4

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Time of last update: 2025-04-16 13:11:08

Author of last update: Jstuelk