argH

argH
168

argininosuccinate lyase

Locus
BSU_29440
Molecular weight
51.76 kDa
Isoelectric point
4.86
Protein length
461 aaSequenceBlast
Gene length
1386 bpSequenceBlast
Function
biosynthesis of arginine
Product
argininosuccinate lyase
Essential
no
E.C.
4.3.2.1
Synonyms
argH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0165 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,011,751  3,013,136
The protein
Catalyzed reaction/ biological activity
2-(Nω-L-arginino)succinate --> fumarate + L-arginine (according to UniProt)
Protein family
lyase 1 family (according to UniProt)
Structure
1TJ7 (PDB) (from Escherichia coli, 47% identity, 64% similarity) PubMed
O34858 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
argG-argH
Description
PubMed
Regulation
repressed by arginine (AhrC)
induced in the absence of RnpM PubMed
Regulatory mechanism
AhrC: repression, in ahrC regulon
RnpM: unknown, PubMed, in rnpM regulon
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argG→argH

2025-03-05 01:06:45

Jstuelk

107

1c207e814f33fb3d04dcc49c4c9da5b8d9da0a69

F891D79BBED99BE9F3E6174F94CE2EF8EDC02509

Biological materials
Mutant
GP1688 Δ(argG-argH)::aphA3, available in Jörg Stülke's lab
GP3712 (argH::aphA3 trpC2) available in the Stülke lab
BKE29440 (argH::erm  trpC2) available at BGSC and in Jörg Stülke's lab GP3925,  PubMed, upstream reverse: _UP1_AAATCGGCCTCCCCAAAGCT,  downstream forward: _UP4_TAAAATTTCCTTCATAAAAT
BKK29440 (argH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAATCGGCCTCCCCAAAGCT,  downstream forward: _UP4_TAAAATTTCCTTCATAAAAT
Expression vectors
pGP3764: expression of Strep-argH by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
pGP3779: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
References
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571789ADA1F7B9DE0F9E205E2DEB9C9166F6C312

Page visits: 2948

Time of last update: 2025-04-10 10:56:59

Author of last update: Jstuelk