Difference between revisions of "PrkD"
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02030 prkD] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02030 prkD] | ||
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| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PrkD PrkD] | ||
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 6.359 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 6.359 | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' autophosphorylated on Thr-174 (''in vitro'') {{PubMed|20389117}} | * '''Modification:''' autophosphorylated on Thr-174 (''in vitro'') {{PubMed|20389117}} | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** [[AbrB]]-[[PrkD]] {{PubMed|24731262}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ybdM_223219_223989_-1 prkD] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ybdM_223219_223989_-1 prkD] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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=References= | =References= | ||
| − | <pubmed> 20389117 12406230 21304896</pubmed> | + | <pubmed> 20389117 12406230 21304896 24731262</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:33, 16 April 2014
- Description: protein kinase D
| Gene name | prkD |
| Synonyms | ybdM |
| Essential | no |
| Product | protein kinase D |
| Function | unknown |
| Gene expression levels in SubtiExpress: prkD | |
| Interactions involving this protein in SubtInteract: PrkD | |
| MW, pI | 29 kDa, 6.359 |
| Gene length, protein length | 768 bp, 256 aa |
| Immediate neighbours | ybzI, ybdN |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
protein modification, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU02030
Phenotypes of a mutant
Database entries
- BsubCyc: BSU02030
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: protein kinase domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification: autophosphorylated on Thr-174 (in vitro) PubMed
- Effectors of protein activity:
Database entries
- BsubCyc: BSU02030
- Structure:
- UniProt: O31435
- KEGG entry: [2]
- E.C. number: 2.7.11.1
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP578 (aphA3), available in the Stülke lab
- Expression vector:
- pGP390 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP821, available in Stülke lab
- for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1407, available in Stülke lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
