alsD

alsD
168

acetolactate decarboxylase

Locus
BSU_36000
Molecular weight
28.65 kDa
Isoelectric point
4.6
Protein length
255 aaSequenceBlast
Gene length
768 bpSequenceBlast
Function
overflow metabolism
Product
acetolactate decarboxylase
Essential
no
E.C.
4.1.1.5
Synonyms
alsD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3527 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,708,799  3,709,566
Phenotypes of a mutant
reduced acetoin production PubMed
impaired biofilm development PubMed
The protein
Catalyzed reaction/ biological activity
(2S)-2-acetolactate + H+ --> (R)-acetoin + CO2 (according to UniProt)
Protein family
alpha-acetolactate decarboxylase family (single member, according to UniProt)
Structure
5XNE (PDB) PubMed
Q04777 (AlphaFold)
Modification
phosphorylated on ser/ thr/ tyr PubMed, in vitro phosphorylated by PrkC on Ser-88 PubMed
Expression and Regulation
Operons
Genes
alsS-alsD
Description
PubMed
Regulation
induction by acetate (AlsR) PubMed
expression is heterogeneous PubMed
Regulatory mechanism
AlsR: activation, in the presence of acetate PubMed, in alsR regulon
Rex: repression, if the ratio NADH2/NAD is high PubMed, in rex regulon
stringent response: positive regulation, due to presence of adenines at +1 and +2 positions of the transcript PubMed, in stringent response
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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alsS→alsD

2025-03-27 04:41:19

Jstuelk

89

95ece8b3897d9d4f146bcd915028c1350cc16fc4

AFF8B3E4EA08E038C6FE3D86BD1815CA5F988C58

Biological materials
Mutant
BKE36000 (alsD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTCCCTTCACTCCTT,  downstream forward: _UP4_TAAAAGAAAAAAAGAAAGCC
BKK36000 (alsD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTCCCTTCACTCCTT,  downstream forward: _UP4_TAAAAGAAAAAAAGAAAGCC
Expression vectors
for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP822, available in Jörg Stülke's lab, PubMed
References
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Page visits: 5994

Time of last update: 2025-04-06 01:53:36

Author of last update: Melvin.boenninger