comFC

comFC
168

late competence gene, resembles type 1 phosphoribosyl transferases

Locus
BSU_35450
Molecular weight
26.26 kDa
Isoelectric point
8.85
Protein length
229 aaSequenceBlast
Gene length
690 bpSequenceBlast
Function
nucleotide scavenging
Product
unknown
Essential
no
Synonyms
comFC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1040 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,641,125  3,641,814
The protein
Protein family
comF/gntX family (single member, according to UniProt)
Structure
P39147 (AlphaFold)
Modification
phosphorylated on Arg-74 PubMed
Localization
localizes to one or both cell poles PubMed
Expression and Regulation
Operons
Genes
comFA-comFB-comFC-yvyF-flgM-flgN-flgK-flgL-yviE-fliW-csrA
Description
PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
ComK: activation, PubMed, in comK regulon
DegU: activation, DegU-P, PubMed, in degU regulon
ScoC: repression, PubMed, in scoC regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
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comFA→csrA

2025-03-28 01:21:10

Jstuelk

156

F13A7084BCCB15345BC26C03AF15EE753EFDF196

11180672E46D33F7D747E58441ECB10AEE7F8D8B

Biological materials
Mutant
GP4447 (trpC2 ΔcomFC::neo), available in Jörg Stülke's lab
BKE35450 (comFC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCGAATCACATAATAAAC,  downstream forward: _UP4_TAAATGATTCTGTTTTTATG
BKK35450 (comFC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCGAATCACATAATAAAC,  downstream forward: _UP4_TAAATGATTCTGTTTTTATG
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
References
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04D9D70C0BCFDCBD00B5156908C348135080A9C2

Page visits: 4734

Time of last update: 2025-04-07 04:45:06

Author of last update: Robert.warneke