mnmM

mnmM
168

SAM-dependent class I 5-aminomethyl-2-thiouridine methyltransferase

Locus
BSU_30490
Molecular weight
21.14 kDa
Isoelectric point
5.56
Protein length
Gene length
Function
tRNA modification at U34
Product
SAM-dependent class I 5-aminomethyl-2-thiouridine methyltransferase
Essential
no
Synonyms
ytqB, MnmM

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0275 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,120,964 → 3,121,548
The protein
Catalyzed reaction/ biological activity
modification of tRNA at U34, conversion of 5-aminomethyl-(2-thio)uridine to 5-methylaminomethyl-(2-thio)uridine PubMed
Protein family
Methyltransferase superfamily (according to UniProt)
SAM PubMed
Structure
4PON (PDB), apoenzyme PubMed
4POO (PDB), complex with SAM PubMed
8H1B (PDB), MnmM from S. aureus complexed with SAM and tRNA anti-codon stem loop
Expression and Regulation
Operons
Genes
Description
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mnmLmnmM

2025-01-25 05:03:27

Jstuelk

88

41ea300e4ac8df30571e89f138c6ef23f3dc8087

D4FF20B78E329CEF138104936884C6F02065E25F

Biological materials
Mutant
MGNA-A165 (ytqB::erm), available at the NBRP B. subtilis, Japan
BKE30490 (ΔmnmM::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_GTAAGGAAGAATTTTCTTCA,  downstream forward: _UP4_GTCGCCATCGAAAAAAAAGC
BKK30490 (ΔmnmM::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_GTAAGGAAGAATTTTCTTCA,  downstream forward: _UP4_GTCGCCATCGAAAAAAAAGC
References
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Time of last update: 2025-01-25 22:35:58

Author of last update: Jstuelk