ecfT

ecfT
168

trans-membrane T component of ECF transporters

Locus
BSU_01470
Molecular weight
29.54 kDa
Isoelectric point
10.18
Protein length
265 aaSequenceBlast
Gene length
798 bpSequenceBlast
Function
uptake of micronutrients
Product
ECF transporter (membrane-spanning protein)
Essential
no
Synonyms
ybaF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0619 (Galperin et al., 2021)

Gene
Coordinates
152,130  152,927
Phenotypes of a mutant
defective in swarming motility (reduced rate of colony expansion) PubMed
The protein
Protein family
energy-coupling factor EcfT family (single member, according to UniProt)
Structure
4HUQ (PDB) (EcfA1-EcfA2-EcfT-FolT complex of Lactobacillus brevis), PubMed
P70972 (AlphaFold)
Localization
cell membrane PubMed
Biological materials
Mutant
GP4703 (trpC2 ΔecfT::neo), available in Jörg Stülke's lab PubMed
GP4772 (trpC2 ΔecfT::lox72), available in Jörg Stülke's lab
MGNA-B945 (ybaF::erm), available at the NBRP B. subtilis, Japan
BKE01470 (ecfT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTGCCGATAATCATGCTGT,  downstream forward: _UP4_TTCTTAAGGGCTTAGAGGTG
BKK01470 (ecfT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTGCCGATAATCATGCTGT,  downstream forward: _UP4_TTCTTAAGGGCTTAGAGGTG
BP1149 (ecfA1-ecfA2-ecfT::cat trp+) available at Jörg Stülke's and Fabian Commichau's labs
References
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Time of last update: 2025-04-04 12:55:09

Author of last update: Jstuelk