DgcW

• Description: diguanylate cyclase and potential phosphodiesterase
  
  

Gene name	dgcW
Synonyms	ykoW
Essential	no
Product	diguanylate cyclase and potential phosphodiesterase
Function	synthesis of c-di-GMP
Gene expression levels in SubtiExpress: dgcW
Metabolic function and regulation of this protein in SubtiPathways: DgcW
MW, pI	84 kDa, 6.823
Gene length, protein length	2247 bp, 749 aa
Immediate neighbours	ykoV, ykoX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU13420

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • synthesis of c-di-GMP from two molecules of GTP PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • contains a N-terminal PAS domain PubMed
  • contains a central GGDEF domain and a C-terminal EAL domain PubMed
  • aa 290-735 are similar to E. coli CsrD (18% identity, 43% similarity)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34311

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: dgcW PubMed

• Expression browser: dgcW PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP850 (ykoW::cat), available in Jörg Stülke's lab
  • BP140 (ykoW::ermC) available in Fabian Commichau's lab
  • BP142 (ykoW-ykoV-ligD::kan) available in Fabian Commichau's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• Antibody: