dgcK

dgcK
168

diguanylate cyclase

Locus
BSU_09120
Molecular weight
40.53 kDa
Isoelectric point
8.57
Protein length
359 aaSequenceBlast
Gene length
1080 bpSequenceBlast
Function
synthesis of c-di-GMP
Product
diguanylate cyclase
Essential
no
Synonyms
dgcK, yhcK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG2199 (Galperin et al., 2021)

Gene
Coordinates
985,734 → 986,813
The protein
Catalyzed reaction/ biological activity
synthesis of c-di-GMP from two molecules of GTP PubMed
Domains
six transmembrane helices at the N-terminus (according to UniProt?)
contains a C-terminal GGDEF domain (aa 223-357) PubMed
Structure
2WB4 (PDB) (the C-terminal GGDEF domain, PleD from Caulobacter vibrioides, 44% identity)
P54595 (AlphaFold)
Localization
cell membrane at cell poles and septa, probably close to YdaK PubMed
present at a single site in the cell membrane PubMed
Expression and Regulation
Operons
Genes
dgcK
Description
PubMed
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dgcK

2025-04-01 20:14:12

Jstuelk

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6fb719aec180a2a9f53e5c3e742c0ce6b95965a6

6808C49BF2474851D3EBF58E0AEC973BBEF8D090

Biological materials
Mutant
MGNA-A656 (yhcK::erm), available at the NBRP B. subtilis, Japan
GP848 (''dgcK::ermC''), available in Jörg Stülke's lab PubMed
BKE09120 (ΔdgcK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATATTATCACCCTTATA,  downstream forward: _UP4_TGAATTCAATGTTCGAATTC
BKK09120 (ΔdgcK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATATTATCACCCTTATA,  downstream forward: _UP4_TGAATTCAATGTTCGAATTC
References
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Time of last update: 2025-04-07 04:57:54

Author of last update: Jstuelk