satA

satA
168

streptothricine acetyl-transferase

Locus
BSU_40740
Molecular weight
20.25 kDa
Isoelectric point
5.83
Protein length
173 aaSequenceBlast
Gene length
522 bpSequenceBlast
Function
resistance to streptothricine
Product
streptothricine acetyl-transferase
Essential
no
Synonyms
satA, yyaR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0456 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,185,160  4,185,681
Phenotypes of a mutant
sensitive to streptothricine PubMed
The protein
Catalyzed reaction/ biological activity
acetylation of streptothricin at the expense of acetyl-CoA PubMed
acetyl-CoA + streptothricin D --> CoA + H+ + Nβ-acetylstreptothricin D (according to UniProt)
acetyl-CoA + streptothricin F --> CoA + H+ + Nβ-acetylstreptothricin F (according to UniProt)
Protein family
Acetyltransferase family (according to UniProt)
Domains
N-acetyltransferase domain (aa 21-173) (according to UniProt)
Structure
3PP9 (PDB) (complex with acetyl-CoA, from B. anthracis, 35% identity, 57% similarity)
P37506 (AlphaFold)
Kinetic information
Km (streptothricine): 1 M PubMed
Km (acetyl-CoA): 107 M PubMed
Biological materials
Mutant
MGNA-B856 (yyaR::erm), available at the NBRP B. subtilis, Japan
BKE40740 (satA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCAATCTCCGCCAATC,  downstream forward: _UP4_TAATTGATTATAAAACCTCA
BKK40740 (satA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCAATCTCCGCCAATC,  downstream forward: _UP4_TAATTGATTATAAAACCTCA
References
Research papers
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Time of last update: 2025-04-03 04:47:23

Author of last update: Melvin.boenninger