walI

walI
168

negative effector of WalK, controls cell wall metabolism

Locus
BSU_40380
Molecular weight
32.44 kDa
Isoelectric point
5.28
Protein length
280 aaSequenceBlast
Gene length
843 bpSequenceBlast
Function
control of cell wall metabolism
Product
negative effector of WalK
Essential
no
Synonyms
walI, yycI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4853 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,149,667 → 4,150,509
Phenotypes of a mutant
growth defect at high temperature, lysis at 49°C, this can be suppressed by mutations that reduce WalK autokinase activity, by inactivation of lytE, or by overexpreesion of IseA or PdaC PubMed
The protein
Structure
2O3O (PDB), \tPubMed
Q45612 (AlphaFold)
Localization
cell membrane (according to UniProt), spotty close to the membrane PubMed
Expression and Regulation
Operons
Genes
walR-walK-walH-walI-walJ-htrC
Description
PubMed
Regulation
htrC transcript expressed during sporulation PubMed
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walR→htrC

2025-03-16 20:03:42

ghost

162

4e891bb3dceb8eda2e5ad4f9ed646a611afbe349

32BB8D419E54714C8925B07FBBCF691251CA4145

Biological materials
Mutant
MGNA-B825 (yycI::erm), available at the NBRP B. subtilis, Japan
BKE40380 (ΔwalI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGCAACGATGAAGATTGATT,  downstream forward: _UP4_TGAAAATAATGGAGTGAGAC
BKK40380 (ΔwalI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGCAACGATGAAGATTGATT,  downstream forward: _UP4_TGAAAATAATGGAGTGAGAC
References
Reviews
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Original Publications
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EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D

Page visits: 2731

Time of last update: 2025-04-02 12:53:00

Author of last update: Melvin.boenninger