walJ
168

5'-3' double-stranded DNA exonuclease, coordination of cell division with DNA replication

Locus

BSU_40370

Molecular weight

29.77 kDa

Isoelectric point

5.5

Protein length

264 aaSequenceBlast

Gene length

795 bpSequenceBlast

Function

coordination of cell division with DNA replication

Product

5'-3' double-stranded DNA exonuclease

Essential

E.C.

3.-.-.-

Synonyms

walJ, yycJ

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1235 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

4,148,851 → 4,149,645

Phenotypes of a mutant

the mutant divides over unsegregated chromosomes more frequently than wild-type cells, and this phenotype is exacerbated when DNA replication is inhibited PubMed

increased sensitivity to a narrow spectrum of cephalosporin antibiotics PubMed

disruption of the corresponding gene in B. anthracis results in a mutator phenotype PubMed

improved survival after DNA replication arrest imposed by inhibition of PolC activity PubMed

The protein

Catalyzed reaction/ biological activity

5'-3' exonuclease activity at the 5' termini and at nicks of double-stranded DNA PubMed

the enzymatic activity of WalJ directly or indirectly affects cell wall metabolism and is required for accurate coordination of cell division with DNA replication PubMed

Protein family

predicted member of the metallo-β-lactamase superfamily

Cofactors

Mn(II) PubMed

Structure

C0SP91 (AlphaFold)

Expression and Regulation

Operons

Genes

walR-walK-walH-walI-walJ-htrC

Description

PubMed