epeE

epeE
168

radical SAM epimerase, modification of EpeX

Locus
BSU_40170
Molecular weight
37.06 kDa
Isoelectric point
5.86
Protein length
319 aaSequenceBlast
Gene length
960 bpSequenceBlast
Function
control of LiaR-LiaS activity
Product
radical SAM epimerase
Essential
no
Synonyms
yydG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0535 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,126,481  4,127,440
The protein
Catalyzed reaction/ biological activity
epimerization of Val-36 and Ile-44 of EpeX to the D-form PubMed
Protein family
radical SAM enzymes PubMed
Cofactors
two 4Fe-4S clusters PubMed
SAM PubMed
Structure
8AI1 (PDB) PubMed
Q45595 (AlphaFold)
Expression and Regulation
Operons
Genes
epeX-epeE-epeP-epeA-epeB
Description
PubMed
Regulation
repressed during logarithmic growth (AbrB) PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
Rok: repression, PubMed, in rok regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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epeX→epeB

2025-03-30 23:00:09

Jstuelk

101

078981D5A4F256B0FA27157DFABCDDE534EC3306

BE1247045A413B93C3150C913C43B4E1B8098926

Biological materials
Mutant
MGNA-B810 (yydG::erm), available at the NBRP B. subtilis, Japan
BKE40170 (epeE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAAATATCCCCCAACAA,  downstream forward: _UP4_TATCCTTATATGGAAAAATA
BKK40170 (epeE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAAATATCCCCCAACAA,  downstream forward: _UP4_TATCCTTATATGGAAAAATA
References
Reviews
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Original Publications
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25FC87037222084ABE318813959101016FEB49DF

Page visits: 3265

Time of last update: 2025-04-03 10:49:07

Author of last update: Jstuelk