aag

aag
168

hypoxanthine-DNA glycosylase, general stress protein, required for protection against paraquat stress

Locus
BSU_38620
Molecular weight
21.98 kDa
Isoelectric point
6.12
Protein length
196 aaSequenceBlast
Gene length
591 bpSequenceBlast
Function
DNA repair, survival of stress conditions
Product
hypoxanthine-DNA glycosylase
Essential
no
Synonyms
aag, yxlJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2094 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,964,278  3,964,868
Phenotypes of a mutant
increased sensitivity of sporulating cells to the genotoxic effects of MMS PubMed
The protein
Catalyzed reaction/ biological activity
binds to 8-oxoG, abasic, and RNA base DNA lesions PubMed
removes hypoxanthine, 3-alkylated purines, and 1,N6-ethenoadenine from DNA; hypoxanthine and 1,N6-ethenoadenine are the preferred substrates PubMed
protects sporulating cells from cytotoxic and genotoxic effects of MMS PubMed
Protein family
DNA glycosylase MPG family (single member, according to UniProt)
Structure
3QI5 (PDB) (the human enzyme in complex with DNA, 33% identity) PubMed
P94378 (AlphaFold)
Localization
forespore PubMed
Expression and Regulation
Operons
Genes
aag-yxzF
Description
PubMed
Regulation
expressed late during sporulation in the forespore SigG PubMed
Sigma factors
SigB: sigma factor, PubMed, in sigB regulon
SigG: sigma factor, PubMed, in sigG regulon
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aag→yxzF

2025-03-26 07:52:33

ghost

91

2e8b672555f6a425e250bf03a974e94656637507

136A388E58BFB19B9852E8EAEF40166A3E0CCE89

Biological materials
Mutant
MGNA-B756 (yxlJ::erm), available at the NBRP B. subtilis, Japan
BKE38620 (aag::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGAATCGACCTCTCCTTT,  downstream forward: _UP4_TAGCATGTTTAAGGAAGAGG
BKK38620 (aag::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGAATCGACCTCTCCTTT,  downstream forward: _UP4_TAGCATGTTTAAGGAAGAGG
References
Reviews
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Original Publications
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305DCA94A69BCF64E377F142722A035871A3B824

Page visits: 2597

Time of last update: 2025-04-04 07:19:30

Author of last update: Jstuelk